Inoculation and growth conditions for high-cell-density expansion of mammalian neural stem cells in suspension bioreactors.

@article{Kallos1999InoculationAG,
  title={Inoculation and growth conditions for high-cell-density expansion of mammalian neural stem cells in suspension bioreactors.},
  author={Michael S Kallos and Leo A. Behie},
  journal={Biotechnology and bioengineering},
  year={1999},
  volume={63 4},
  pages={473-83}
}
Inoculation and growth conditions for the large-scale expansion of mammalian neural stem cells (NSC) have been determined. We examined suspension culture bioreactors of murine NSC, and concluded that the oxygen level should be kept high (20%), and the osmolarity of the medium should be kept low (below 400 mOsm/kg). The pH of the medium was found to have a large effect on cell proliferation, and the best growth characteristics were obtained within an optimum pH range of 7. 1 to 7.5. The… CONTINUE READING
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