Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi

@article{Vierheilig1998InkAV,
  title={Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi},
  author={Vierheilig and Coughlan and Wyss and Pich{\'e}},
  journal={Applied and environmental microbiology},
  year={1998},
  volume={64 12},
  pages={5004-7}
}
We developed a reliable, inexpensive, and simple method for staining arbuscular-mycorrhizal fungal colonizations in root tissues. Apart from applications in research, this nontoxic, high-quality staining method also could be of great utility in teaching exercises. After adequate clearing with KOH, an ink-vinegar solution successfully stained all fungal structures, rendering them clearly visible.