Injection of recombinant adenovirus for delivery of genetically encoded calcium indicators into astrocytes of the cerebellar cortex.

@article{Kuhn2011InjectionOR,
  title={Injection of recombinant adenovirus for delivery of genetically encoded calcium indicators into astrocytes of the cerebellar cortex.},
  author={Bernd Kuhn and Tycho M Hoogland and Samuel S-H Wang},
  journal={Cold Spring Harbor protocols},
  year={2011},
  volume={2011 10},
  pages={1217-23}
}
The cerebellar cortex contains two astrocyte types: the Bergmann glia of the molecular layer and the velate protoplasmic astrocytes of the granule cell layer. In vivo, these cell types generate both subcellular calcium transients and trans-glial calcium waves. This protocol outlines a method for in vivo calcium imaging in cerebellar astrocytes, using the… CONTINUE READING