Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis

@article{OToole1998InitiationOB,
  title={Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis},
  author={G. O’Toole and R. Kolter},
  journal={Molecular Microbiology},
  year={1998},
  volume={28}
}
Populations of surface‐attached microorganisms comprising either single or multiple species are commonly referred to as biofilms. Using a simple assay for the initiation of biofilm formation (e.g. attachment to an abiotic surface) by Pseudomonas fluorescens strain WCS365, we have shown that: (i) P. fluorescens can form biofilms on an abiotic surface when grown on a range of nutrients; (ii) protein synthesis is required for the early events of biofilm formation; (iii) one (or more… Expand
Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development
TLDR
The isolation and characterization of mutants of Pseudomonas aeruginosa PA14 defective in the initiation of biofilm formation on an abiotic surface, polyvinylchloride (PVC) plastic are reported and evidence that microcolonies form by aggregation of cells present in the monolayer is presented. Expand
Rhamnolipid Surfactant Production Affects Biofilm Architecture in Pseudomonas aeruginosa PAO1
TLDR
It is proposed that the maintenance of biofilm architecture represents a previously unrecognized step in the development of these microbial communities, and evidence that surfactants may be able to maintain open channels by affecting cell-cell interactions and the attachment of bacterial cells to surfaces is provided. Expand
Streptococcus gordonii Biofilm Formation: Identification of Genes that Code for Biofilm Phenotypes
TLDR
Molecular analyses of biofilm-defective mutants of S. gordonii Challis showed that some of the genes required for biofilm formation are involved in signal transduction, peptidoglycan biosynthesis, and adhesion, which are associated with quorum sensing, osmoadaptation, andAdhesion functions in oral streptococci. Expand
Identification of psl, a Locus Encoding a Potential Exopolysaccharide That Is Essential for Pseudomonas aeruginosa PAO1 Biofilm Formation
TLDR
It is discovered that one group of genes, designated psl, are important for biofilm initiation and understanding psl-encoded exopolysaccharide expression and protection in biofilms will provide insight into the pathogenesis of P. aeruginosa in cystic fibrosis and other infections involving biofilmms. Expand
Genes involved in matrix formation in Pseudomonas aeruginosa PA14 biofilms
TLDR
Results suggest that the pel genes are responsible for the production of a glucose‐rich matrix material required for the formation of biofilms by P. aeruginosa PA14. Expand
The sporulation transcription factor Spo0A is required for biofilm development in Bacillus subtilis
TLDR
It is reported here that the soil bacterium Bacillus subtilis forms biofilms, and mutations in abrB suppressed the biofilm defect of a spo0A mutant, indicating that AbrB negatively regulates at least one gene that is required for the transition from a monolayer of attached cells to a mature biofilm. Expand
Genetic determinants of Pseudomonas aeruginosa biofilm establishment.
TLDR
Screening of the comprehensive Harvard Pseudomonas aeruginosa PA14 mutant library for mutants exhibiting an altered biofilm phenotype found that the establishment of a biofilm requires regulatory components that are involved in survival under microaerophilic growth conditions, arginine metabolism, alkyl-quinolone signalling, pH homeostasis and the DNA repair system. Expand
SadB Is Required for the Transition from Reversible to Irreversible Attachment during Biofilm Formation by Pseudomonas aeruginosa PA14
TLDR
A new class of P. aeruginosa biofilm mutant is reported that defines the transition from reversible to irreversible attachment and is thus required for monolayer formation. Expand
Different, overlapping mechanisms for colonization of abiotic and plant surfaces by Pseudomonas putida.
TLDR
A mutant altered in lipopolysaccharide biosynthesis is impaired in seed and root colonization but seems to initiate attachment to plastic faster than the wild type, and the partial overlap between mechanisms leading to biofilm formation on abiotic and biotic surfaces. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 63 REFERENCES
Green fluorescent protein as a marker for Pseudomonas spp
TLDR
Two plasmids, pSMC2 and pGB5, are particularly useful, because they are stable in the absence of antibiotic selection, they place an undetectable metabolic burden on cells that carry the plasmid, and cells carrying these constructs continue to fluoresce even after 7 days in culture without the addition of fresh nutrients. Expand
Development of an Adhesion Assay and Characterization of an Adhesion-Deficient Mutant of Pseudomonas fluorescens
TLDR
A sand column adhesion assay was developed which distinguishes the adhesion abilities of a number of pseudomonads isolated from fine sandy loam, finding that less Pf0-5 than wild-type bacteria were recovered, suggesting a decreased ability to persist in soil. Expand
Characterization of Pseudomonas aeruginosa fliO, a gene involved in flagellar biosynthesis and adherence
TLDR
To characterize genes involved in non-pilus-mediated adherence, transposon mutants of the nonpiliated strain P. aeruginosa PAK-NP that are unable to bind to cells or mucins were isolated and sequence analysis indicated that this open reading frame encodes a putative protein homolog of both Escherichia coli FliO and Erwinia carotovora subsp. Expand
Siderophores and outer membrane proteins of antagonistic, plant-growth-stimulating, root-colonizing Pseudomonas spp
TLDR
The results showed that all strains produce hydroxamate-type siderophores which are thought to be the major factor responsible for antagonistic activity, and it is unlikely that the Fe3+ uptake system of the antagonistic strains is usually plasmid encoded. Expand
Tn5 Insertion Mutants of Pseudomonas fluorescens Defective in Adhesion to Soil and Seeds
TLDR
It is indicated that the same mutations that affect binding to sand and soil also affect adhesion to plant seeds. Expand
Effect of Motility on Surface Colonization and Reproductive Success of Pseudomonas fluorescens in Dual-Dilution Continuous Culture and Batch Culture Systems
TLDR
Bacterial motility conveyed a selective advantage during surface colonization even in aqueous-phase systems not dominated by laminar flow, as confirmed by dual-dilution continuous culture results. Expand
Characterization of a Pseudomonas aeruginosa gene cluster involved in pilus biosynthesis and twitching motility: sequence similarity to the chemotaxis proteins of enterics and the gliding bacterium Myxococcus xanthus
  • A. Darzins
  • Biology, Medicine
  • Molecular microbiology
  • 1994
The type 4 pill of Pseudomonas aeruginosa are important cell‐associated virulence factors that play a crucial role in mediating (i) bacterial adherence to, and colonization of, mucosal surfaces, (ii)Expand
Phenazine antibiotic biosynthesis in Pseudomonas aureofaciens 30-84 is regulated by PhzR in response to cell density
TLDR
The results indicate that PhzR is a member of a two-component sensor-regulator family with known or predicted carboxy-terminal DNA-binding domains which regulates gene expression in response to environmental and cell density signals. Expand
Direct evidence for the involvement of extracellular proteins in the adhesion of Azospirillum brasilense.
TLDR
The correlation between protein concentration at the cell surface or at the support surface and adhesion density, under different experimental conditions, provides a direct demonstration of the involvement of extracellular proteins in the adhesion of A. brasilense to inert surfaces. Expand
Siderophore activity of pyoverdin for Pseudomonas aeruginosa
TLDR
Activity, iron binding, and the stimulation of bacterial iron transport indicated that pyoverdin can function as a siderophore for P. aeruginosa, which may be related to the pathogenicity of this bacterium. Expand
...
1
2
3
4
5
...