Initiation of a Sarcocystis neurona expressed sequence tag (EST) sequencing project: a preliminary report.

@article{Howe2001InitiationOA,
  title={Initiation of a Sarcocystis neurona expressed sequence tag (EST) sequencing project: a preliminary report.},
  author={Daniel K. Howe},
  journal={Veterinary parasitology},
  year={2001},
  volume={95 2-4},
  pages={233-9}
}
To accelerate genetic and molecular characterization of Sarcocystis neurona, the primary causative agent of equine protozoal myeloencephalitis (EPM), a sequencing project has been initiated that will generate approximately 7000-8000 expressed sequence tags (ESTs) from this apicomplexan parasite. Poly(A)(+) RNA was isolated from culture-derived S. neurona merozoites, and a cDNA library was constructed in a unidirectional lambda phage cloning vector. Sixty phage clones were randomly picked from… CONTINUE READING
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