Initiation of RNA decay in Escherichia coli by 5' pyrophosphate removal.

  title={Initiation of RNA decay in Escherichia coli by 5' pyrophosphate removal.},
  author={Helena {\vC}ele{\vs}nik and Atilio Deana and Joel G. Belasco},
  journal={Molecular cell},
  volume={27 1},
The common belief that endonucleolytic cleavage is the initial, rate-determining step of mRNA decay in Escherichia coli fails to explain the influence of 5' termini on the half-lives of primary transcripts. We have re-examined the initial events of RNA degradation in that organism by devising an assay to probe the 5' phosphorylation state of RNA and by employing a self-cleaving hammerhead ribozyme to investigate the degradative consequences of an unphosphorylated 5' end. These studies have… CONTINUE READING

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