Initiation of Protein Synthesis from the A Site of the Ribosome

  title={Initiation of Protein Synthesis from the A Site of the Ribosome},
  author={Joan E Wilson and Tatyana V. Pestova and Christopher U. T. Hellen and Peter Sarnow},
Positioning of the translation initiation complex on mRNAs requires interaction between the anticodon of initiator Met-tRNA, associated with eIF2-GTP and 40S ribosomal subunit, and the cognate start codon of the mRNA. We show that an internal ribosome entry site located in the genome of cricket paralysis virus can form 80S ribosomes without initiator Met-tRNA, eIF2, or GTP hydrolysis, with a CCU triplet in the ribosomal P site and a GCU triplet in the A site. P-site mutagenesis revealed that… CONTINUE READING
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