Initiation of DNA synthesis by microtubule disruption in quiescent rat 3Y1 cells.

@article{Shinohara1989InitiationOD,
  title={Initiation of DNA synthesis by microtubule disruption in quiescent rat 3Y1 cells.},
  author={Yoshihiko Shinohara and Eisuke Nishida and Hideaki Sakai},
  journal={European journal of biochemistry},
  year={1989},
  volume={183 2},
  pages={275-80}
}
Disruption of cytoplasmic microtubules by colchicine, colcemid or vinblastine induced the initiation of DNA synthesis and cell division in quiescent cultures of rat embryo fibroblast cell line, 3Y1, in the absence of growth factors. The microtubule-disruption-induced DNA synthesis was so marked that we could initiate analysis of the mechanism. Incubation of quiescent 3Y1 cells with high concentrations of vinblastine or those of vinblastine plus colcemid, which formed large tubulin paracrystals… CONTINUE READING

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