Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs

@article{Blow1986InitiationOD,
  title={Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs},
  author={J. Blow and R. Laskey},
  journal={Cell},
  year={1986},
  volume={47},
  pages={577-587}
}
We demonstrate that cell-free extracts prepared from activated eggs of X. laevis by a method similar to that of Lohka and Masui initiate and complete semiconservative DNA replication of sperm nuclei and plasmid DNA. The efficiency of replication is comparable to that in the intact egg. Under optimal conditions 70%-100% of nuclei, and up to 38% of naked DNA molecules replicate completely. Genuine initiation of replication occurs rather than elongation of preformed primers or priming of… Expand
DNA replication in nucleus-free Xenopus egg extracts.
DNA replication in cell-free extracts from Xenopus laevis.
Study of DNA replication in Drosophila using cell free in vitro system
Chromosome replication in cell-free systems from Xenopus eggs.
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