Initiation of DNA replication by the dnaG protein.

  title={Initiation of DNA replication by the dnaG protein.},
  author={Edmund W. Benz and Danny F Reinberg and R. Vicu{\~n}a and Jerard Hurwitz},
  journal={The Journal of biological chemistry},
  volume={255 3},
Highly purified preparations of dnaG protein from Escherichia coli prime minus strand synthesis of phage alpha 3 DNA in vitro. This protein synthesizes primer oligonucleotides which may be composed of ribonucleotide or deoxyribonucleotide moieties or both. The presence of deoxyribonucleotide moieties in the chain limits primer chain length; this effect occurs even when ribonucleoside triphosphates are included in the priming reaction. The dnaG protein can use ADP in place of ATP. Primer… CONTINUE READING

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