Initiating a crystallographic analysis of recombinant (S)-2-hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis.

@article{Cameron2005InitiatingAC,
  title={Initiating a crystallographic analysis of recombinant (S)-2-hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis.},
  author={Scott Cameron and Karen McLuskey and Rachel Chamberlayne and Irene Hallyburton and William N. Hunter},
  journal={Acta crystallographica. Section F, Structural biology and crystallization communications},
  year={2005},
  volume={61 Pt 5},
  pages={534-6}
}
The oxirane (1R,2S)-1,2-epoxypropylphosphonic acid (fosfomycin) is a natural product antibiotic produced in Streptomyces wedmorensis by the metal-ion-dependent (S)-2-hydroxypropylphosphonic acid epoxidase. This epoxidase is highly unusual since it has no requirement for a haem prosthetic group. The gene encoding the enzyme, fom4, has been cloned and a highly efficient recombinant source of the enzyme established. Two different crystal forms, tetragonal and hexagonal, have been obtained. The… CONTINUE READING