Initial stages of influenza hemagglutinin-induced cell fusion monitored simultaneously by two fluorescent events: cytoplasmic continuity and lipid mixing

@article{Sarkar1989InitialSO,
  title={Initial stages of influenza hemagglutinin-induced cell fusion monitored simultaneously by two fluorescent events: cytoplasmic continuity and lipid mixing},
  author={Debi Prasad Sarkar and Stephen S. Morris and O. Eidelman and Joshua Zimmerberg and Robert Blumenthal},
  journal={The Journal of Cell Biology},
  year={1989},
  volume={109},
  pages={113 - 122}
}
We have monitored the mixing of both aqueous intracellular and membrane-bound fluorescent dyes during the fusion of human red blood cells to influenza hemagglutinin-expressing fibroblasts using fluorescence spectroscopy and low light, image-enhanced video microscopy. The water-soluble fluorescent dye, N-(7-nitrobenzofurazan-4-yl)taurine, was incorporated into intact human red blood cells. The fluorescence of the dye in the intact red blood cell was partially quenched by hemoglobin. The lipid… CONTINUE READING