Initial phase of infection with Tyzzer's organism in cultured mouse hepatocytes.

Abstract

The initial phase of infection with Tyzzer's organisms in cultured mouse hepatocytes was observed using indirect immunofluorescence (IF) and a plaque assay. The organisms adhered poorly to aldehyde- or acetone-fixed cells, but once adhered to host cells, whether methanol-fixed or unfixed, they were not removed by methanol or acetone. By the IF as well as the plaque assay, both of which discriminated intra- and extracellularly located organisms, the number of cell-associated organisms increased linearly up to 3 h post-inoculation. The number of intra-cellular organisms increased rapidly in the first 1 h, followed by linear increase at a much lower rate. Similar results were obtained by the plaque assay.

Cite this paper

@article{Kawamura1989InitialPO, title={Initial phase of infection with Tyzzer's organism in cultured mouse hepatocytes.}, author={Sadafumi Kawamura and Taeko Mitsuoka and Kenji Fujiwara}, journal={Journal of general microbiology}, year={1989}, volume={135 7}, pages={2099-106} }