Initial Test of the Benchmark Chemical Approach for Predicting Microbial Transformation Rates in Aquatic Environments

  title={Initial Test of the Benchmark Chemical Approach for Predicting Microbial Transformation Rates in Aquatic Environments},
  author={Thomas D. Newton and David K. Gattie and David L. Lewis},
  journal={Applied and Environmental Microbiology},
  pages={288 - 291}
  • T. D. Newton, D. Gattie, David L. Lewis
  • Published 1990
  • Medicine, Chemistry
  • Applied and Environmental Microbiology
Using 2,4-dichlorophenoxyacetic acid methyl ester (2,4-DME) as a benchmark chemical, we determined relative pseudo-first-order rate coefficients for the butoxyethyl ester of 2,4-dichlorophenoxyacetic acid (2,4-DBE), methyl parathion, and methyl-3-chlorobenzoate in a diversity of microbial samples, including water, sediment, biofilm, and floating microbial mats collected from a laboratory mesocosm as well as from streams, lakes, and wetlands in Georgia and Florida. The decreasing order of… Expand
Quantitative assessment of the effects of metals on microbial degradation of organic chemicals
Effects of heavy metals on aerobic microbial degradation rates of organic chemicals appeared to be considerably more inhibitory to the biodegradation of an organic chemical than high concentrations of microbially toxic organics studied previously. Expand
The effect of algal blooms on the disappearance of phenol in a small forest pond
During the periods of “blooms” of the green algae in the pond the concentrations of inorganic nutrients were comparatively high and the phenol-degrading bacteria likely were not limited by these nutrients, in contrast to the periods-of-bloom of the blue–green algae on the other hand in the reservoir. Expand
Inoculum Standardization for Biodegradability Tests
To obtain standardized inoculum with constant characteristics for biodegradability test, Actizym powder was used instead of sludge. A predigestion and stabilization step were introduced to prepareExpand


Predicting 2,4-Dichlorophenoxyacetic Acid Ester Transformation Rates in Periphyton-Dominated Ecosystems
Using batch cultures, transformation rate coefficients for microbial transformation of 2,4-dichlorophenoxyacetic acid butoxyethyl ester in periphyton-dominated ecosystems were determined and mean second-order rate coefficients were used to predict 2, 4-DBE transformation rates in microcosms having diverse chemical and biological environments. Expand
Prediction of Substrate Removal Rates of Attached Microorganisms and of Relative Contributions of Attached and Suspended Communities at Field Sites
  • David L. Lewis, D. Gattie
  • Medicine, Chemistry
  • Applied and environmental microbiology
  • 1988
Under conditions of mass transport limitation, the contributions of attached microorganisms relative to total microbial activities at various field sites were related to the ratio of water velocity, U, and depth, D, showing that historical definitions of ecosystems according to flow and depth characteristics are also valid for describing the process-related structure of ecosystems. Expand
Rates of Transformation of Methyl Parathion and Diethyl Phthalate by Aufwuchs Microorganisms
  • David L. Lewis, H. W. Holm
  • Medicine, Biology
  • Applied and environmental microbiology
  • 1981
Using batch cultures, transformation rates for low concentrations of two toxicants are determined by aufwuchs, aquatic microbial growth attached to submerged surfaces or suspended in streamers or mats, for potential use in a mathematical model capable of predicting the transport and fate of chemicals in aquatic systems. Expand
Kinetic Concepts for Measuring Microbial Rate Constants: Effects of Nutrients on Rate Constants
The use of nutrient-amended waters could be a useful tool for estimating second-order rate constants for an expanded number of compounds, and this technique would provide a larger data base for predicting the behavior of xenobiotic compounds in the environment by using currently available mathematical models. Expand
Microbial Transformation of Esters of Chlorinated Carboxylic Acids
The microbial rate constants of the carboxylic acid esters with a fixed aromatic moiety increased with an increasing length of alkyl substituents, and the regression coefficient for the linear relationships between log k(b) and log K(ow) was high for group 1 compounds, indicating that these parameters correlated well. Expand
Laboratory procedure for determining substrate removal rate coefficients for biofilms
Abstract Although numerous techniques have been standardized for assessing the metabolic activities of free-living (suspendent) microorganisms, little corresponding methodoloty has been developed forExpand