Inhibitory effect of the plant-extract osthole on L-type calcium current in NG108-15 neuronal cells.

@article{Wu2002InhibitoryEO,
  title={Inhibitory effect of the plant-extract osthole on L-type calcium current in NG108-15 neuronal cells.},
  author={Sheng-Nan Wu and Y. Lo and Chien-Chih Chen and H. F. Li and H. Chiang},
  journal={Biochemical pharmacology},
  year={2002},
  volume={63 2},
  pages={
          199-206
        }
}
The effects of osthole, a coumarin isolated from Cnidium monnieri (L.) Cusson, on ionic currents in a mouse neuroblastoma and rat glioma hybrid cell line, NG105-18, were investigated with the aid of the whole-cell voltage-clamp technique. Osthole (0.3-100 microM) caused an inhibition of voltage-dependent L-type Ca(2+) current (I(Ca,L)) in a concentration-dependent manner. Osthole produced no change in the overall shape of the current-voltage relationship of I(Ca,L). The IC(50) value of the… Expand
Inhibitory action of L-type Ca2+ current by paeoniflorin, a major constituent of peony root, in NG108-15 neuronal cells.
TLDR
It is indicated that paeoniflorin can block L-type Ca2+ channels in NG108-15 cells in a mechanism unlinked to the binding to adenosine receptors. Expand
Mechanism of osthole inhibition of vascular Ca(v)1.2 current.
TLDR
Osthole is a vascular Ca(v)1.2 channel antagonist stabilizing the channel in its inactivated state, which may account for the systolic blood pressure reduction induced by the drug in animal models of hypertension and points to osthole as a lead for the development of novel antihypertensive agents. Expand
Effect of arvanil (N-arachidonoyl-vanillyl-amine), a nonpungent anandamide-capsaicin hybrid, on ion currents in NG108-15 neuronal cells.
TLDR
The channel-blocking properties of arvanil may, at least in part, contribute to the underlying mechanisms by which it affects neuronal or neuroendocrine function. Expand
Differential effects of quercetin, a natural polyphenolic flavonoid, on L‐Type calcium current in pituitary tumor (GH3) cells and neuronal NG108‐15 cells
TLDR
The effects of quercetin on ion currents may, at least in part, contribute to the underlying mechanisms through which it affects neuronal or neuroendocrine function. Expand
Neuroprotective effects of osthole pretreatment against traumatic brain injury in rats
TLDR
Results suggested that osthole had a neuroprotective effect against TBI, and the protection may be associated with its antioxidative and antiapoptotic functions. Expand
Mitigation of H2O2-Induced Mitochondrial-Mediated Apoptosis in NG108-15 Cells by Novel Mesuagenin C from Mesua kunstleri (King) Kosterm
TLDR
Results indicated that mesuagenin C 3 could protect NG108-15 cells against H2O2-induced apoptosis by increasing intracellular GSH level, aggrandizing Δψm, and modulating apoptotic signalling pathway through Bcl-2 family and caspase-3/7 and -9. Expand
Coumarsabin hastens C-type inactivation gating of voltage-gated K⁺ channels.
TLDR
It is reported that coumarsabin, a coumarin derivative isolated from Juniperus Sabina, could hasten C-type inactivation and thus cause block of Kv channels in neuronal NG108-15 cells and Kv1.2 channels heterologously expressed in lung epithelial H1355 cells. Expand
Inhibitory Effects of Columbianadin on Nociceptive Behaviors in a Neuropathic Pain Model, and on Voltage-Gated Calcium Currents in Dorsal Root Ganglion Neurons in Mice
TLDR
The results suggest that CBN, as well as OST, might inhibit neuropathic pain behaviors through an inhibition of T- and L-type calcium currents in nociceptive DRG neurons. Expand
Osthole: A Review on Its Bioactivities, Pharmacological Properties, and Potential as Alternative Medicine
TLDR
The pharmacological properties of osthole are summarized and an overview of the underlying mechanisms are given, which showcase its potential as a multitarget alternative medicine. Expand
Induction of cell differentiation in human leukemia U-937 cells by 5-oxygenated-6,7-methylenedioxycoumarins from Pterocaulon polystachyum.
TLDR
Treatment with pure 5-methoxy-6,7-methylenedioxycoumarin (C1) and 5-3-methyl-2-butenyloxy (C2) might have a potential therapeutic role in the management of leukemia. Expand
...
1
2
...

References

SHOWING 1-10 OF 26 REFERENCES
Ionic mechanisms of tetrandrine in cultured rat aortic smooth muscle cells.
TLDR
Results indicate that tetrandrine directly inhibits the voltage-dependent L-type Ca2+ current in vascular smooth muscle cells, which may predominantly contribute to the vasodilatory actions of tetr andrine. Expand
Antiproliferative effect in rat vascular smooth muscle cells by osthole, isolated from Angelica pubescens.
TLDR
It is suggested that osthole is a selective antiproliferative agent in vascular smooth muscle cells and is due to the increase in cyclic AMP and cyclic GMP contents. Expand
Calcineurin involvement in the regulation of high‐threshold Ca2+ channels in NG108–15 (rodent neuroblastoma × glioma hybrid) cells
TLDR
Findings indicate that PP2B activity does not influence the expression of HVA Ca2+ channels, but modulates their function by Ca2-dependent dephosphorylation, with the major effect being on N‐type VOCCs. Expand
Effect of the plant-extract osthole on the relaxation of rabbit corpus cavernosum tissue in vitro.
TLDR
The data suggested that osthole possesses a relaxant effect on rabbit corpus cavernosal tissues which is attributable to the release of NO from sinusoidal endothelium and to the potentiation of the cGMP and/or cAMP signal mediating relaxation of cavernosal smooth muscle by inhibiting phosphodiesterase. Expand
The relaxant action of osthole isolated from Angelica pubescens in guinea-pig trachea
TLDR
It is concluded that osthole exerts a nonspecific relaxant effect on the trachealis by inhibiting the cAMP and cGMP phosphodiesterases. Expand
Effect of capsaicin on membrane currents in cultured vascular smooth muscle cells of rat aorta.
TLDR
Results suggest that inhibition of the voltage-dependent L-type Ca2+ channel is involved in the capsaicin-induced relaxation of the vascular smooth muscle, whereas capsaicIn-induced inhibition of voltage- dependent K+ channels might produce an increase in cell excitability. Expand
cADP-ribose potentiates cytosolic Ca2+ elevation and Ca2+ entry via L-type voltage-activated Ca2+ channels in NG108-15 neuronal cells.
TLDR
The results suggest that cADPR functions on ryanodine receptors as a direct agonist and also interacts with L-type VACCs as an indirect agonist, i.e. via a retrograde signal. Expand
Vasorelaxation of rat thoracic aorta caused by osthole isolated from Angelica pubescens.
TLDR
It is concluded that osthole relaxes rat thoracic aorta by virtue of its Ca(2+)-channel blocking properties and by elevating cGMP levels in vascular smooth muscle. Expand
Dihydropyridine‐sensitive and omega‐conotoxin‐sensitive calcium channels in a mammalian neuroblastoma‐glioma cell line.
TLDR
The results indicate that two classes of Ca2+ channels contribute to the HVA currents of this cell line, and the DHP‐sensitive channel is more apt to generate Ca 2+ spikes and Ca2- plateau potentials than the omega CgTX‐ sensitive channel. Expand
Effects of osthole on isolated guinea pig heart atria.
TLDR
Ost was similar to, but much less potent than Ver in inhibiting the isolated guinea pig atria, and showed only slight inhibitory effect on post-rest potentiation. Expand
...
1
2
3
...