Inhibitory effect of filamin A on invasion and metastasis of human colon carcinoma cell line SW480 in vitro

@inproceedings{Guiying2011InhibitoryEO,
  title={Inhibitory effect of filamin A on invasion and metastasis of human colon carcinoma cell line SW480 in vitro},
  author={Wang Gui-ying},
  year={2011}
}
Objective To explore the inhibitory effect of Filamin A(FLNa) on invasion and metastasis of human colon carcinoma cell line SW480 and investigate its mechanisms.Methods Expressing vector pcDNA3.1/V5-His-TOPO/ FLNa was transfected into SW480 cells by lipofectamine protocols.After G418 selection,the SW480/FLNa cells expressing FLNa stably were obtained.Expression of FLNa in tumor cells was confirmed by RT-PCR and Western blot.RT-PCR assay and Western blot were also employed to detect the… CONTINUE READING