Inhibitory effect of ethanol on growth and solute accumulation by Saccharomyces cerevisiae as affected by plasma-membrane lipid composition

  title={Inhibitory effect of ethanol on growth and solute accumulation by Saccharomyces cerevisiae as affected by plasma-membrane lipid composition},
  author={D. Susan Thomas and Anthony H. Rose},
  journal={Archives of Microbiology},
Incorporation of ethanol (1.0 or 1.25 M) into exponential-phase cultures of Saccharomyces cerevisiae NCYC 366 growing anaerobically in a medium supplemented with ergosterol and an unsaturated fatty acid caused a retardation in growth rate, which was greater when the medium contained oleic rather than linoleic acid. Ethanol incorporation led to an immediate drop in growth rate, and ethanol-containing cultures grew at the slower rate for at least 10 h. Incorporation of ethanol (0.5 M) into… Expand
Effect of cellular inositol content on ethanol tolerance of Saccharomyces cerevisiae in sake brewing.
It was suggested that the yeast cellular inositol level is one of the important factors which contribute to the high ethanol tolerance implied by the increased cell viability in the presence of ethanol. Expand
Relationship between ethanol tolerance and fatty acyl composition of Saccharomyces cerevisiae
Results demonstrated that S. cerevisiae became more resistant to ethanol with an increase in the degree of unsaturation and that membrane fluidity could be an important determinant of ethanol tolerance. Expand
Effect of growth rate on ethanol tolerance ofSaccharomyces cerevisiae
AbstractΔ5,7Saccharomyces cerevisiae cells growing in chemostat at a specific growth rate of 0.075/h exhibited higher ethanol tolerance measured as ethanol-induced death and anaerobic growthExpand
Determining the effects of inositol supplementation and the opi1 mutation on ethanol tolerance of Saccharomyces cerevisiae.
It is concluded that the introduction of an opi1 mutation in yeast results in an inherent increase in PI levels and constitutive biosynthesis of inositol that, in turn, will reduce the cost of supplementing inositl into the media to achieve a higher ethanol tolerance. Expand
Ethanol tolerance ofSaccharomyces cerevisiae and its relationship to lipid content and composition
The change in the percentage of free fatty acids was shown to be the controlling factor in ethanol tolerance and it was shown that growth is completely prevented at 14% ethanol. Expand
Synergistic effects of ethanol and temperature on yeast mitochondria
At temperatures lower than 37°C, the ethanol inhibition constant (Ki) for growth or fermentation inrho+ cells of theSaccharomyces cerevisiae strain S288C was always higher than in rho− mutants, and both strains were equally inhibited by ethanol. Expand
Variations of two pools of glycogen and carbohydrate in Saccharomyces cerevisiae grown with various ethanol concentrations
Elevated level of cell-surface α-glucans in yeast grown with ethanol, as measured by using amyloglucosidase treatment, confirms the correlation between ethanol and glycogen and suggests that membrane-bound insoluble glycogen might play a protective role in combating ethanol stress. Expand
Physiological properties and plasma membrane composition of Saccharomyces cerevisiae grown in sequential batch culture and in the presence of surfactants
The kinetics of sphaeroplast production revealed that recycled cells were more resistant to lytic enzymes and that culture of the cells in the presence of the surfactants had no effect on cell wall lysis. Expand
Role of mitochondria in ethanol tolerance of Saccharomyces cerevisiae
The presence of active mitochondria and oxidative metabolism is shown to be essential to maintain low inhibition levels by ethanol of the growth rate (μ), fermentation rate (v) or respiration rateExpand
Alterations in fatty acid composition and trehalose concentration ofSaccharomyces brewing strains in response to heat and ethanol shock
It is suggested that unsaturated fatty acids and high concentrations of intracellular trehalose may protect the cells from the inhibitory effects of heat and ethanol shock. Expand


Environmentally-induced changes in the neutral lipids and intracellular vesicles of Saccharomyces cerevisiae and Kluyveromyces fragilis
Both yeasts synthesized highly saturated triacylglycerols, relatively unsaturated phospholipids, and esterified sterols with an intermediate degree of unsaturation irrespective of the conditions under which they were grown. Expand
Osmotic lysis of sphaeroplasts from Saccharomyces cerevisiae grown anaerobically in media containing different unsaturated fatty acids.
Summary: Saccharomyces cerevisiae NCYC 366 was grown anaerobically under conditions that induce a requirement for a sterol and an unsaturated fatty acid. In media containing ergosterol and eitherExpand
Plasma-Membrane lipid composition and ethanol tolerance inSaccharomyces cerevisiae
Populations of cells suspended anaerobically in buffered (pH 4.5) M ethanol remained viable to a greater extent when their plasma membranes were enriched in linoleyl rather than oleyl residues irrespective of the nature of the sterol enrichment, while populations grown in the presence of this sterol and palmitoleic acid were more resistant to ethanol. Expand
Effect of ethanol and other alcohols on the transport of amino acids and glucose by everted sacs of rat small intestine.
The rapid inhibition of amino acid transport, the ease of reversibility of this inhibition, and the relatively small increase in back-diffusion, indicate that ethanol inhibits active transport under the conditions of these experiments. Expand
Fragility of plasma membranes in Saccharomyces cerevisiae enriched with different sterols
Cholesterol-enriched cells contained about 2% more lipid than cells enriched in any of the other sterols, which was largely accounted for by increased contents of triacylglycerols and, to a lesser extent, esterified sterols. Expand
The metabolism of hydroxystearic acids in yeast.
It is now clear that the hydroxy acids or their coenzyme A esters do not as such have the properties of intermediates in the biosynthesis of oleic acid, but hydroxystearic acids can replace oleoic acid as growth factors for anaerobic yeast. Expand
Fragility of the permeability barrier of Escherichia coli.
Abstract An unsaturated fatty acid requiring auxotroph of Escherichia coli was grown with addition of various unsaturated fatty acids. The permeability of the cells for erythritol appeared to beExpand
Multiplicity of the amino acid permeases in Saccharomyces cerevisiae. IV. Evidence for a general amino acid permease.
Kinetic and genetic evidences are presented to show that, in addition to specific amino acid permeases, Saccharomyces cerevisiae has a general amino acid permease which catalyzes the transport ofExpand
Adaptation of membrane lipids to alcohols
  • L. Ingram
  • Medicine, Biology
  • Journal of bacteriology
  • 1976
The results indicate that Escherichia coli cells radically change their fatty acid composition when grown in the presence of alcohols, which represents an adaptive membrane alteration compensating for the direct physicochemical interaction ofcohols with the membrane. Expand
Cell Membrane Transport: Principles and Techniques
Structural Aspects.- 1. Composition and Structure of Cell Membranes.- 1.1. Cell Envelopes and Membranes.- 1.2. Chemical Composition of the Plasma Membrane.- 1.3. Fine Structure of the PlasmaExpand