Inhibitory effect of cannabichromene, a major non-psychotropic cannabinoid extracted from Cannabis sativa, on inflammation-induced hypermotility in mice.

@article{Izzo2012InhibitoryEO,
  title={Inhibitory effect of cannabichromene, a major non-psychotropic cannabinoid extracted from Cannabis sativa, on inflammation-induced hypermotility in mice.},
  author={Angelo A Izzo and Raffaele Capasso and Gabriella Aviello and Francesca Borrelli and Barbara Romano and Fabiana Piscitelli and Laura E Gallo and Francesco Capasso and Pierangelo Orlando and Vincenzo Di Marzo},
  journal={British journal of pharmacology},
  year={2012},
  volume={166 4},
  pages={1444-60}
}
BACKGROUND AND PURPOSE Cannabichromene (CBC) is a major non-psychotropic phytocannabinoid that inhibits endocannabinoid inactivation and activates the transient receptor potential ankyrin-1 (TRPA1). Both endocannabinoids and TRPA1 may modulate gastrointestinal motility. Here, we investigated the effect of CBC on mouse intestinal motility in physiological and pathological states. EXPERIMENTAL APPROACH Inflammation was induced in the mouse small intestine by croton oil. Endocannabinoid… CONTINUE READING

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Inhibitory effect of cannabichromene , a major non - psychotropic cannabinoid extracted from Cannabis sativa , on inflammation - induced hypermotility in mice .
Endocannabinoid ( anandamide and 2-arachidonoyl glycerol ) , palmitoylethanolamide and oleoylethanolamide levels were measured by liquid chromatography - mass spectrometry ; TRPA1 and cannabinoid receptors were analysed by quantitative RT - PCR ; upper gastrointestinal transit , colonic propulsion and whole gut transit were evaluated in vivo ; contractility was evaluated in vitro by stimulating the isolated ileum , in an organ bath , with ACh or electrical field stimulation ( EFS ) .
Inhibitory effect of cannabichromene , a major non - psychotropic cannabinoid extracted from Cannabis sativa , on inflammation - induced hypermotility in mice .
Ex vivo CBC did not change endocannabinoid levels , but it altered the mRNA expression of TRPA1 and cannabinoid receptors .
Endocannabinoid ( anandamide and 2-arachidonoyl glycerol ) , palmitoylethanolamide and oleoylethanolamide levels were measured by liquid chromatography - mass spectrometry ; TRPA1 and cannabinoid receptors were analysed by quantitative RT - PCR ; upper gastrointestinal transit , colonic propulsion and whole gut transit were evaluated in vivo ; contractility was evaluated in vitro by stimulating the isolated ileum , in an organ bath , with ACh or electrical field stimulation ( EFS ) .
Croton oil administration was associated with decreased levels of anandamide ( but not 2-arachidonoyl glycerol ) and palmitoylethanolamide , up - regulation of TRPA1 and CB₁ receptors and down - regulation of CB₂ receptors .
Croton oil administration was associated with decreased levels of anandamide ( but not 2-arachidonoyl glycerol ) and palmitoylethanolamide , up - regulation of TRPA1 and CB₁ receptors and down - regulation of CB₂ receptors .
Endocannabinoid ( anandamide and 2-arachidonoyl glycerol ) , palmitoylethanolamide and oleoylethanolamide levels were measured by liquid chromatography - mass spectrometry ; TRPA1 and cannabinoid receptors were analysed by quantitative RT - PCR ; upper gastrointestinal transit , colonic propulsion and whole gut transit were evaluated in vivo ; contractility was evaluated in vitro by stimulating the isolated ileum , in an organ bath , with ACh or electrical field stimulation ( EFS ) .
Endocannabinoid ( anandamide and 2-arachidonoyl glycerol ) , palmitoylethanolamide and oleoylethanolamide levels were measured by liquid chromatography - mass spectrometry ; TRPA1 and cannabinoid receptors were analysed by quantitative RT - PCR ; upper gastrointestinal transit , colonic propulsion and whole gut transit were evaluated in vivo ; contractility was evaluated in vitro by stimulating the isolated ileum , in an organ bath , with ACh or electrical field stimulation ( EFS ) .
Ex vivo CBC did not change endocannabinoid levels , but it altered the mRNA expression of TRPA1 and cannabinoid receptors .
Endocannabinoid ( anandamide and 2-arachidonoyl glycerol ) , palmitoylethanolamide and oleoylethanolamide levels were measured by liquid chromatography - mass spectrometry ; TRPA1 and cannabinoid receptors were analysed by quantitative RT - PCR ; upper gastrointestinal transit , colonic propulsion and whole gut transit were evaluated in vivo ; contractility was evaluated in vitro by stimulating the isolated ileum , in an organ bath , with ACh or electrical field stimulation ( EFS ) .
Endocannabinoid ( anandamide and 2-arachidonoyl glycerol ) , palmitoylethanolamide and oleoylethanolamide levels were measured by liquid chromatography - mass spectrometry ; TRPA1 and cannabinoid receptors were analysed by quantitative RT - PCR ; upper gastrointestinal transit , colonic propulsion and whole gut transit were evaluated in vivo ; contractility was evaluated in vitro by stimulating the isolated ileum , in an organ bath , with ACh or electrical field stimulation ( EFS ) .
Endocannabinoid ( anandamide and 2-arachidonoyl glycerol ) , palmitoylethanolamide and oleoylethanolamide levels were measured by liquid chromatography - mass spectrometry ; TRPA1 and cannabinoid receptors were analysed by quantitative RT - PCR ; upper gastrointestinal transit , colonic propulsion and whole gut transit were evaluated in vivo ; contractility was evaluated in vitro by stimulating the isolated ileum , in an organ bath , with ACh or electrical field stimulation ( EFS ) .
Inhibitory effect of cannabichromene , a major non - psychotropic cannabinoid extracted from Cannabis sativa , on inflammation - induced hypermotility in mice .
Endocannabinoid ( anandamide and 2-arachidonoyl glycerol ) , palmitoylethanolamide and oleoylethanolamide levels were measured by liquid chromatography - mass spectrometry ; TRPA1 and cannabinoid receptors were analysed by quantitative RT - PCR ; upper gastrointestinal transit , colonic propulsion and whole gut transit were evaluated in vivo ; contractility was evaluated in vitro by stimulating the isolated ileum , in an organ bath , with ACh or electrical field stimulation ( EFS ) .
Ex vivo CBC did not change endocannabinoid levels , but it altered the mRNA expression of TRPA1 and cannabinoid receptors .
Endocannabinoid ( anandamide and 2-arachidonoyl glycerol ) , palmitoylethanolamide and oleoylethanolamide levels were measured by liquid chromatography - mass spectrometry ; TRPA1 and cannabinoid receptors were analysed by quantitative RT - PCR ; upper gastrointestinal transit , colonic propulsion and whole gut transit were evaluated in vivo ; contractility was evaluated in vitro by stimulating the isolated ileum , in an organ bath , with ACh or electrical field stimulation ( EFS ) .
Ex vivo CBC did not change endocannabinoid levels , but it altered the mRNA expression of TRPA1 and cannabinoid receptors .
Endocannabinoid ( anandamide and 2-arachidonoyl glycerol ) , palmitoylethanolamide and oleoylethanolamide levels were measured by liquid chromatography - mass spectrometry ; TRPA1 and cannabinoid receptors were analysed by quantitative RT - PCR ; upper gastrointestinal transit , colonic propulsion and whole gut transit were evaluated in vivo ; contractility was evaluated in vitro by stimulating the isolated ileum , in an organ bath , with ACh or electrical field stimulation ( EFS ) .
Endocannabinoid ( anandamide and 2-arachidonoyl glycerol ) , palmitoylethanolamide and oleoylethanolamide levels were measured by liquid chromatography - mass spectrometry ; TRPA1 and cannabinoid receptors were analysed by quantitative RT - PCR ; upper gastrointestinal transit , colonic propulsion and whole gut transit were evaluated in vivo ; contractility was evaluated in vitro by stimulating the isolated ileum , in an organ bath , with ACh or electrical field stimulation ( EFS ) .
Endocannabinoid ( anandamide and 2-arachidonoyl glycerol ) , palmitoylethanolamide and oleoylethanolamide levels were measured by liquid chromatography - mass spectrometry ; TRPA1 and cannabinoid receptors were analysed by quantitative RT - PCR ; upper gastrointestinal transit , colonic propulsion and whole gut transit were evaluated in vivo ; contractility was evaluated in vitro by stimulating the isolated ileum , in an organ bath , with ACh or electrical field stimulation ( EFS ) .
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