Inhibitory effect of acidic pH on OmpC porin: wild-type and mutant studies.

By use of the patch clamp technique, we have compared the electrophysiological signature of OmpC porin channels at neutral and acidic pH. The perfusion of pH 5.4 buffer to the periplasmic side of excised patches promoted the closure or block of approximately 20% of the open porins present in the patch without changes in their single channel conductance… CONTINUE READING