Inhibitory effect of D-glucosamine on glycolysis in bovine retina.

@article{Tesoriere1975InhibitoryEO,
  title={Inhibitory effect of D-glucosamine on glycolysis in bovine retina.},
  author={Giovanni Tesoriere and Renza Vento and Giuseppe Calvaruso},
  journal={Biochimica et biophysica acta},
  year={1975},
  volume={385 1},
  pages={
          58-67
        }
}
1. The effects of glucosamine concentration on the size of the lactate pool, on the levels of ATP, ADP, AMP and on the radioactivity incorporation from [1-14-C] glucosamine into lactate, N-acetylglucosamine and glucosamine-6-P were studied using whole bovine retinas. 2. The radioactive lactate, evaluated in relation to glucosamine molarity, after a modest initial increase, diminishes significantly. On the contrary the N-acetyl [1-14-C] glucosamine, the [1-14-C] glucosamine-6-P and, consequently… Expand
Inhibitory effects of D-glucosamine on the metabolism of embryonal and adult retina of chicken.
TLDR
The utilization of the aminosugar is more efficient in embryonal than in adult retina, and d -Glucose is ineffective in the reversal of these effects. Expand
Uridine enhances the cytotoxic effect of D-glucosamine in rat C6 glioma cells.
TLDR
The findings suggest a connection between the accumulation of UDP-N-acetylhexosamines in the cells and the appearance of D-glucosamine cytotoxicity. Expand
Effects of long-term exposure to glucosamine and mannosamine on aggrecan degradation in articular cartilage.
TLDR
It is indicated that continuous presence of amino sugars is required to protect cartilage from stimulated loss of aggrecan and inhibited degradation of radiolabeled and chemical levels of Aggrecan. Expand
Glucosamine reduces anabolic as well as catabolic processes in bovine chondrocytes cultured in alginate.
TLDR
It is suggested that the claimed structure modifying effects of GlcN are more likely based on protection against ECM degradation than new ECM production. Expand
High doses of glucosamine-HCl have detrimental effects on bovine articular cartilage explants cultured in vitro.
TLDR
It is shown that pharmacological doses of glucosamine induce a broad impairment in the metabolic activity of bovine chondrocytes, leading to cell death, and particular attention should be addressed to the experimental model, the doses and the length of treatment. Expand
Excessive Hexosamines Block the Neuroprotective Effect of Insulin and Induce Apoptosis in Retinal Neurons*
  • Makoto Nakamura, A. Barber, +4 authors T. Gardner
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 2001
TLDR
The results suggest that the excessive glucose flux through the HBP may direct retinal neurons to undergo apoptosis in a bimodal fashion; i.e. via perturbation of the neuroprotective effect of insulin mediated by Akt and via induction of apoptosis possibly by altered glycosylation of proteins. Expand
Inhibitory effect of D-glucosamine on protein and nucleic acids biosynthesis in bovine retina.
TLDR
D -Glucose and d -mannose are very effective in reversing the inhibition of protein, RNA and DNA biosynthesis in bovine retina and Uridine reverses partially the glucosamine inhibition ofprotein biosynthesis. Expand
Short-term treatment with glucosamine hydrochloride specifically downregulates hypoxia-inducible factor-1α at the protein level in YD-8 human tongue cancer cells.
TLDR
It was identified that HIF-1α protein downregulation induced by short-term GS-HCl treatment was blunted by exogenous administration of the citric acid cycle metabolites citrate and 2-oxoglutarate, but not the glycolytic end byproducts pyruvate and lactate. Expand
Physiological effects of oral glucosamine on joint health: current status and consensus on future research priorities
TLDR
The available evidence for beneficial effects of GlcN on joint health is summarized and new insight is proposed into the design of future clinical trials aimed at identifying beneficial physiological effect of glucosamine on joint tissues. Expand
Effects of D-glucosamine on the UTP pool and on nucleic acid synthesis in embryonal retina of chicken.
TLDR
D -glucosamine lowers in embryonal retinal of chicken the content of UTP and causes a reduction of RNA synthesis and a reduction in RNA synthesis. Expand
...
1
2
...

References

SHOWING 1-10 OF 14 REFERENCES
Metabolism of glucosamine in bovine retina
Abstract 1. 1. The metabolism of [1-14C]glucosamine had been investigated either in retinal bovine homogenates or in whole retinas. 2. 2. The trichloracetic acid-soluble fractions, obtained fromExpand
The effect of D-glucosamine on the adenine and uridine nucleotides of sarcoma 180 ascites tumor cells.
TLDR
Investigation of the effect of d-glucosamine on the metabolism of adenine and uridine nucleotides in Sarcoma 180 ascites tumor cells found no decrease in the intracellular ATP level was observed in ascites cells exposed to glucosamine in the presence of 10 mm glucose. Expand
Biosynthesis of UDP N-acetylhexosamine in bovine retina.
TLDR
The metabolism of amino sugars in the retina would appear to be much more active than in the pigment epithelial cells; this would indicate that the greater part of the synthesis of mucopoly-saccharides of the interstitial matrix takes place in the eye. Expand
The effect of N-acetylglucosamine and glucosamine on carbohydrate metabolism in rat liver slices.
  • R. Spiro
  • Medicine, Biology
  • The Journal of biological chemistry
  • 1958
TLDR
The metabolism of glucose and fructose by liver slices was studied in the presence of varying concentrations of either N-acetylglucosamine or glucosamine, shown to be competitive inhibitors of glucose phosphorylation by a group of nonspecific hexokinases present in a number of animal tissues and microorganisms. Expand
Inhibitory effect of D-glucosamine and other sugars on the biosynthesis of protein, ribonucleic acid, and deoxyribonucleic acid in normal and neoplastic tissues.
TLDR
The effect of exogenous glucosamine in vitro on the incorporation of the labeled precursors into normal tissues was much less than that into neoplastic tissues. Expand
Transformation fo glucosamine to glycogen and lactate by ascites tumor cells
Abstract 1. 1. Ascites tumors, including Yoshida sarcoma, when incubated in vitro , phosphorylate exogenous glucosamine readily and transform a considerable part of the glucosamine 6-phosphate formedExpand
Studies on the relationships between insulin, glucosamine, and glucose in rat diaphragms.
One approach toward the elucidation of the mechanism of insulin action is to study the effect of insulin on glucose analogues. Studies of this type have been carried out in this laboratory by use ofExpand
Tetrose diphosphate, a specific inhibitor of glyceraldehyde 3-phosphate dehydrogenase.
TLDR
A study of the conditions leading to the formation of inhibitor revealed that it was rapidly formed from glycolaldehyde 2-phosphate in 1 N NaOH at room temperature andolation and analysis of the inhibitor showed it to be a tetrose diphosphate. Expand
Properties of yeast phosphofructokinase.
  • A. Ramaiah
  • Biology, Medicine
  • Biochimica et biophysica acta
  • 1970
TLDR
The yeast phosphofructokinase prepared by the method described here was less sensitive to ATP inhibition than was the enzyme in the crude fraction and could be prevented by diluting the enzyme and storing it in the presence of ATP, AMP or Fru-6-P but not in the absence of MgCl2 or NH4Cl. Expand
GLUCOSE CATABOLISM OF RABBIT RETINA BEFORE AND AFTER DEVELOPMENT OF VISUAL FUNCTION *
TLDR
The fully developed adult rabbit retina was compared with the young tissue at 5 to 7 days after birth, a time when the retina is not yet functioning and when the rods and cones are still small, growing organelles. Expand
...
1
2
...