Inhibitors of DNA topoisomerase II prevent chromatid separation in mammalian cells but do not prevent exit from mitosis.

@article{Downes1991InhibitorsOD,
  title={Inhibitors of DNA topoisomerase II prevent chromatid separation in mammalian cells but do not prevent exit from mitosis.},
  author={C. Stephen Downes and Ann M. Mullinger and Robert T. Johnson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1991},
  volume={88 20},
  pages={8895-9}
}
DNA topoisomerase II (EC 5.99.1.3) is necessary for chromosome condensation and disjunction in yeast but not for other functions. In mammalian cells, it has been reported to be necessary for progression toward mitosis but not for transit through mitosis. We have found, on the contrary, that specific inhibition of topoisomerase II (but not of topoisomerase I) interferes with mammalian mitotic progression. Metaphase is prolonged, and anaphase separation of chromatids is completely inhibited, in… CONTINUE READING