Inhibitor analysis of synapsis by resolvase.

@article{Flanagan1989InhibitorAO,
  title={Inhibitor analysis of synapsis by resolvase.},
  author={Peter M. Flanagan and Mary C. Finn and Michael Fennewald},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 28},
  pages={
          16892-6
        }
}
Previously, we isolated several inhibitors that block the site-specific recombination reaction mediated by the Tn3-encoded resolvase protein. One class of inhibitors blocks resolvase binding to the recombination (res) sitc, and a second class inhibits synapse formation between resolvase and two directly repeated res sites. In this report, we identify an inhibitor, A20832, that does not inhibit resolvase binding to res, as measured by filter binding, or synapse formation. Inhibition of resolvase… 

References

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TLDR
The Tn3-encoded resolvase protein promotes a site-specific recombination reaction between two directly repeated copies of the recombination site res, and DNase I footprinting revealed that at certain concentrations of A9387 and A1062, resolvent was preferentially bound to site I of res, the site containing the recombinational crossover point.
Isolation and characterization of the Tn3 resolvase synaptic intermediate.
TLDR
It is concluded that the specificity of recombination is achieved by a three‐stage recognition system: binding of resolvase to separate sites, formation of the synaptosome and determination of site orientation from within the complex.
Recombination by resolvase is inhibited by lac repressor simultaneously binding operators between res sites.
TLDR
The result suggests that lac repressor can inhibit resolvase by forming a DNA loop that isolates a res site topologically, and suggests that the structure and topology of the DNA substrate is important in the formation of a synapse between res sites.
Analysis of gamma delta resolvase mutants in vitro: evidence for an interaction between serine-10 of resolvase and site I of res.
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TLDR
The properties of these resolvase mutants with substitutions at position 10 are consistent with this hypothesis and suggest that serine-10 is in close contact with the DNA at site I, and it is shown that a phosphoserine linkage is made to the DNA during recombination.
Isolation and analysis of inhibitors of transposon Tn3 site-specific recombination
TLDR
A genetic bioassay for inhibitors of site-specific recombination by transposon Tn3 resolvase is constructed and identified analogs of A1062 which inhibit.
Cleavage of the site-specific recombination protein gamma delta resolvase: the smaller of two fragments binds DNA specifically.
TLDR
Resolvase has a modular construction analogous to that found for some repressors and activators; its COOH-terminal domain recognizes specific sequences in the DNA and its NH2-terminAL domain mediates protein-protein interactions and probably has the enzymatic activity.
The γδ resolvase induces an unusual DNA structure at the recombinational crossover point
TLDR
The properties of the mutant protein suggest that the induced structural change, which is proposed is a localized kink, is required for recombination.
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TLDR
The data strongly suggest that there are in fact three negative supercoils between synapsed sites; one supercoil is dissolved in the cross-over mechanism, whereas the other two are metamorphosed into the unique catenane interlock.
Site-specific relaxation and recombination by the Tn3 resolvase: Recognition of the DNA path between oriented res sites
TLDR
Because resolvase is a type 1 topoisomerase, it is inferred that it makes the required duplex bDNA breaks of recombination one strand at a time.
Discovery of a predicted DNA knot substantiates a model for site-specific recombination.
TLDR
The mechanism of site-specific genetic recombination mediated by Tn3 resolvase has been investigated by a topological approach and the structure of the knot was as predicted, providing strong evidence for the model and showing the power of the topological method.
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