Inhibition of zebrafish fgf8 pre‐mRNA splicing with morpholino oligos: A quantifiable method for gene knockdown

@article{Draper2001InhibitionOZ,
  title={Inhibition of zebrafish fgf8 pre‐mRNA splicing with morpholino oligos: A quantifiable method for gene knockdown},
  author={Bruce W. Draper and Paul A. Morcos and Charles B Kimmel},
  journal={genesis},
  year={2001},
  volume={30}
}
Using Morpholinos to Control Gene Expression
  • Jon D Moulton
  • Medicine
  • Current protocols in nucleic acid chemistry
  • 2017
TLDR
This work focuses on techniques and background for using Morpholinos, a stable, uncharged, water‐soluble molecules used to block complementary sequences of RNA, preventing processing, read‐through, or protein binding at those sites. Expand
sall4 acts downstream of tbx5 and is required for pectoral fin outgrowth
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It is demonstrated that sall4 and a related gene sall1 act downstream of tbx5 and are required for pectoral fin development and offer explanations for the similarity of individuals with OS and HOS limb defects. Expand
Kupffer's vesicle is a ciliated organ of asymmetry in the zebrafish embryo that initiates left-right development of the brain, heart and gut
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The proposed KV is a transient embryonic `organ of asymmetry' that directs LR development by establishing a directional fluid flow and genetic analysis reveals novel roles for the T-box transcription factor no tail and the Nodal signaling pathway as upstream regulators of lrdr1 expression and KV morphogenesis. Expand
Multiple roles for the Na,K-ATPase subunits, Atp1a1 and Fxyd1, during brain ventricle development.
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Data include the first reported role for Fxyd1 in the developing brain, and indicate that the Na,K-ATPase regulates three aspects of brain ventricle development essential for normal function: formation of a cohesive neuroepithelium, restriction of Neuroepithelial permeability, and production of CSF. Expand
Using Morpholinos to Control Gene Expression
TLDR
This work focuses on techniques and background for using Morpholinos, which are typically used to inhibit translation of mRNA, splicing of pre‐mRNA, and maturation of miRNA, although they can also inhibit other interactions between biological macromolecules and RNA. Expand
Specification of epibranchial placodes in zebrafish
TLDR
A model in which mesoderm-derived Fgf3 and FgF8 signals establish both the EB placodes and the development of the pharyngeal endoderm, the subsequent interaction of which promotes neurogenesis is proposed. Expand
Using Morpholinos to Control Gene Expression
  • Jon D Moulton
  • Biology, Medicine
  • Current protocols in nucleic acid chemistry
  • 2007
TLDR
This work focuses on techniques and background for using Morpholinos, a stable, uncharged, water‐soluble molecules used to block complementary sequences of RNA, preventing processing, read‐through, or protein binding at those sites. Expand
Early requirement for fgf8 function during hindbrain pattern formation in zebrafish
  • E. Wiellette, H. Sive
  • Biology, Medicine
  • Developmental dynamics : an official publication of the American Association of Anatomists
  • 2004
TLDR
An early, nonredundant requirement for fgf8 function in hindbrain patterning is demonstrated in zebrafish, and it is proposed that this recovery is a response to activation of f gf3 and to delayed accumulation of fgF8. Expand
Comparison of molecular marker expression in early zebrafish brain development following chronic ethanol or morpholino treatment
TLDR
The results of this study suggest that while chronic ethanol induces obvious morphological change in brain architecture, many molecular markers of these brain structures are relatively unaffected by ethanol exposure. Expand
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