Inhibition of vitronectin-mediated haptotaxis and haptoinvasion of MG-63 cells by domain 5 (D5(H)) of human high-molecular-weight kininogen and identification of a minimal amino acid sequence.

@article{Kamiyama2001InhibitionOV,
  title={Inhibition of vitronectin-mediated haptotaxis and haptoinvasion of MG-63 cells by domain 5 (D5(H)) of human high-molecular-weight kininogen and identification of a minimal amino acid sequence.},
  author={Fumiaki Kamiyama and Toshinaga Maeda and Takashi Yamane and Yue Hu Li and O Ogukubo and Toshiaki Otsuka and Hisao Ueyama and Shin-ichiro Takahashi and Iwao Ohkubo and Nobuo Matsui},
  journal={Biochemical and biophysical research communications},
  year={2001},
  volume={288 4},
  pages={
          975-80
        }
}
We found that human kinin-free high-molecular-weight kininogen (kf-HK) significantly inhibited vitronectin-mediated migration (haptotaxis) and invasive potentiation (haptoinvasion) of osteosarcoma (MG-63) cells but that HK, LK, the common heavy chain of HK and LK, and the light chain (D6(H)) of HK had no inhibitory effect. Recombinant GST-D5(H) (histidine-rich region of HK) obtained from Escherichia coli. (BL21) also inhibited both haptotaxis and haptoinvasion to about 30% of the control level… CONTINUE READING
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