Inhibition of translational initiation in the yeast Saccharomyces cerevisiae as a function of the stability and position of hairpin structures in the mRNA leader.

@article{Laso1993InhibitionOT,
  title={Inhibition of translational initiation in the yeast Saccharomyces cerevisiae as a function of the stability and position of hairpin structures in the mRNA leader.},
  author={M R Vega Laso and Dahai Zhu and Francis Sagliocco and Andrew J Brown and Mick F Tuite and John G. McCarthy},
  journal={The Journal of biological chemistry},
  year={1993},
  volume={268 9},
  pages={6453-62}
}
A new modular in vivo/in vitro expression system was constructed which facilitates studies of the control and regulation of gene expression in the yeast Saccharomyces cerevisiae. We studied the influence of stem-loop structures inserted into the non-translated leader region upon the steady-state levels and translation of mRNAs bearing the cat gene from the bacterial transposon Tn9. mRNA abundance changed relatively little in response to alterations in the leader sequence and structure, whereas… CONTINUE READING

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