Inhibition of transfected PTEN on human colon cancer.


AIM To study the inhibitory effect of transfected PTEN on LoVo cells. METHODS Human PTEN cDNA was transferred into LoVo cells via lipofectin and PTEN mRNA levels and its expression were analyzed by Western blot and flow cytometry. Before or after transfection, the effects of 5-Fu on inhibiting cell proliferation and inducing apoptosis were measured by… (More)


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