Inhibition of thermal induced protein denaturation of extract/fractions of Withania somnifera and isolated withanolides.

Abstract

This study describes the in vitro inhibition of protein denaturation of extract/fractions of Withania somnifera and isolated withanolides including 20β hydroxy-1-oxo(22R)-witha-2,5,24 trienolide (1), (20R,22R-14α,20α)-dihydroxy-1-oxowitha-2,5,16,24 tetraenolide (2). The results showed that the extract/fractions of the plant evoked profound inhibitory effect on thermal-induced protein denaturation. The chloroform fraction caused the most dominant attenuation of 68% at 500 μg/mL. The bioactivity-guided isolation from chloroform fraction led to the isolation of compounds 1 and 2 that showed profound protein inhibition with 78.05% and 80.43% effect at 500 μg/mL and thus strongly complimented the activity of extract/fractions. In conclusion, extract/fractions of W. somnifera possessed strong inhibition of protein denaturation that can be attributed to these isolated withanolides.

DOI: 10.1080/14786419.2015.1007138

Cite this paper

@article{Khan2015InhibitionOT, title={Inhibition of thermal induced protein denaturation of extract/fractions of Withania somnifera and isolated withanolides.}, author={Murad Khan and Haroon Khan and Abdul Mannan Rauf and Taibi ben Hadda}, journal={Natural product research}, year={2015}, volume={29 24}, pages={2318-21} }