Inhibition of the neutral magnesium-dependent sphingomyelinase by glutathione.

@article{Liu1997InhibitionOT,
  title={Inhibition of the neutral magnesium-dependent sphingomyelinase by glutathione.},
  author={Baolian Liu and Yusuf A. Hannun},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 26},
  pages={16281-7}
}
Sphingomyelin hydrolysis through the activation of sphingomyelinases has become a potentially important signaling pathway with the product ceramide implicated in the regulation of cell growth, differentiation, apoptosis, and inflammatory responses. However, little is known about the regulation of sphingomyelinases. In this study, we show that the magnesium-dependent, neutral pH-optimum and membrane-associated sphingomyelinase (N-SMase) is inhibited, in a dose-dependent manner, by glutathione… CONTINUE READING
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Sphingomyelin hydrolysis through the activation of sphingomyelinases has become a potentially important signaling pathway with the product ceramide implicated in the regulation of cell growth , differentiation , apoptosis , and inflammatory responses .
Finally , treatment of cultured Molt-4 cells with the GSH synthesis inhibitor , L - buthionine-(SR)-sulfoximine , resulted in a time - dependent depletion of GSH , accompanied by an increased hydrolysis of sphingomyelin and production of ceramide .
Sphingomyelin hydrolysis through the activation of sphingomyelinases has become a potentially important signaling pathway with the product ceramide implicated in the regulation of cell growth , differentiation , apoptosis , and inflammatory responses .
Finally , treatment of cultured Molt-4 cells with the GSH synthesis inhibitor , L - buthionine-(SR)-sulfoximine , resulted in a time - dependent depletion of GSH , accompanied by an increased hydrolysis of sphingomyelin and production of ceramide .
Sphingomyelin hydrolysis through the activation of sphingomyelinases has become a potentially important signaling pathway with the product ceramide implicated in the regulation of cell growth , differentiation , apoptosis , and inflammatory responses .
Finally , treatment of cultured Molt-4 cells with the GSH synthesis inhibitor , L - buthionine-(SR)-sulfoximine , resulted in a time - dependent depletion of GSH , accompanied by an increased hydrolysis of sphingomyelin and production of ceramide .
On the other hand , neither dithiothreitol nor beta - mercaptoethanol had any effect on the N - SMase , suggesting that the sulfhydryl in GSH is not required for inhibition of N - SMase .
Sphingomyelin hydrolysis through the activation of sphingomyelinases has become a potentially important signaling pathway with the product ceramide implicated in the regulation of cell growth , differentiation , apoptosis , and inflammatory responses .
Finally , treatment of cultured Molt-4 cells with the GSH synthesis inhibitor , L - buthionine-(SR)-sulfoximine , resulted in a time - dependent depletion of GSH , accompanied by an increased hydrolysis of sphingomyelin and production of ceramide .
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