Inhibition of the in vitro formation of irreversibly sickled cells by cepharanthine.

@article{Ohnishi1983InhibitionOT,
  title={Inhibition of the in vitro formation of irreversibly sickled cells by cepharanthine.},
  author={S. Tsuyoshi Ohnishi},
  journal={British journal of haematology},
  year={1983},
  volume={55 4},
  pages={665-71}
}
A method was developed to prepare irreversibly sickled cells (ISC) in vitro under a physiological condition. By exposing sickle red cells to repeated deoxygenation-reoxygenation cycles for 15 h at 37 degrees C, 20-30% of the red cells formed ISC. These cells were separated from biconcave-shaped cells by a gradient density centrifugation. The percentage of the formation of ISC was determined spectrophotometrically after cells were haemolysed. Cepharanthine, a bisbenzylisoquinoline alkaloid, was… CONTINUE READING

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