• DOI:10.1111/j.1476-5381.1994.tb17022.x
• Corpus ID: 25021880

Inhibition of the glutamate transporter and glial enzymes in rat striatum by the gliotoxin, ocaminoadipate

@article{McBean1994InhibitionOT,
  title={Inhibition of the glutamate transporter and glial enzymes in rat striatum by the gliotoxin, ocaminoadipate},
  author={Gethin J. McBean},
  journal={British Journal of Pharmacology},
  year={1994},
  volume={113}
}

• G. McBean
• Published 1 October 1994
• Biology, Chemistry
• British Journal of Pharmacology

1 The effect of the gliotoxic analogue of glutamate, ocaminoadipate, on the high affinity transport of D‐[3H]‐aspartate into a crude striatal P2 preparation, and on the activity of two enzymes of which glutamate is the substrate has been examined. 2 The L‐isomer of ocaminoadipate competitively inhibited the transport protein, with a Ki value of 192 μm, whereas the D‐isomer of ocaminoadipate was ineffective. The potent convulsant, L‐methionine‐S‐sulphoximine, was also without effect on the activity of the gluatmate transport protein. 3 L‐αAminoadipate was a competitive inhibitor of both glutamine synthetase, and γ‐glutamylcysteine synthetase, with Ki values of 209 μm and 7 mm respectively. Once again, the D‐isomer of ocaminoadipate was a far weaker inhibitor of either enzyme. 4 The results are discussed in terms of the mechanism of action of ocaminoadipate in causing toxicity of glial cells.