Inhibition of the glutamate transporter and glial enzymes in rat striatum by the gliotoxin, ocaminoadipate

@article{McBean1994InhibitionOT,
  title={Inhibition of the glutamate transporter and glial enzymes in rat striatum by the gliotoxin, ocaminoadipate},
  author={Gethin J. McBean},
  journal={British Journal of Pharmacology},
  year={1994},
  volume={113}
}
  • G. McBean
  • Published 1 October 1994
  • Biology, Chemistry
  • British Journal of Pharmacology
1 The effect of the gliotoxic analogue of glutamate, ocaminoadipate, on the high affinity transport of D‐[3H]‐aspartate into a crude striatal P2 preparation, and on the activity of two enzymes of which glutamate is the substrate has been examined. 2 The L‐isomer of ocaminoadipate competitively inhibited the transport protein, with a Ki value of 192 μm, whereas the D‐isomer of ocaminoadipate was ineffective. The potent convulsant, L‐methionine‐S‐sulphoximine, was also without effect on the… 
Visualising the activity of the cystine‐glutamate antiporter in glial cells using antibodies to aminoadipic acid, a selectively transported substrate
  • D. Pow
  • Biology, Chemistry
    Glia
  • 2001
TLDR
This study used the simple strategy of generating an antibody to aminoadipic acid, a selective substrate for the cystine‐glutamate antiporter to suggest that this antiporter might contribute to release of glutamate from glial cells under some pathophysiological conditions.
The glio-toxic mechanism of α-aminoadipic acid on cultured astrocytes
TLDR
It is suggested that A A A is toxic to mitotic astrocytes by interference with protein synthesis needed for cell division by interfering with glutamate release by microglia.
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 30 REFERENCES
The glutamate analogue alpha-aminoadipic acid is taken up by astrocytes before exerting its gliotoxic effect in vitro
TLDR
It is shown thatalpha-AA appears to be taken up by the astrocytes before exerting its cytotoxic effect, and sodium plays its role in alpha-AA-induced gliotoxicity by mediating the transport of the substance.
An In Vivo Model for Studying Function of Brain Tissue Temporarily Devoid of Glial Cell Metabolism: The Use of Fluorocitrate
TLDR
The use of microinjection of 1 nmol of fluorocitrate into the neostria‐tum of the rat to provide a model for studying transmitter amino acid metabolism in brain devoid of glial cell activity is discussed.
Neurotoxicity of L‐Glutamate and DL‐Threo‐3‐Hydroxyaspartate in the Rat Striatum
TLDR
The results indicate that removal, or blockade, of uptake sites for glutamate increase the vulnerability of striatal neurones to the toxic effects of synaptically released glutamate.
Structure, expression, and functional analysis of a Na(+)-dependent glutamate/aspartate transporter from rat brain.
TLDR
The structure and function of a 66-kDa glycoprotein that was purified from rat brain and identified as an L-glutamate/L-aspartate transporter (GLAST) are described and data suggest that GLAST may be involved in the regulation of neurotransmitter concentration in central nervous system.
Cloning and expression of a rat brain L-glutamate transporter
TLDR
An antibody against a glial L-glutamate transporter from rat brain is used to isolate a complemen-tary DNA clone encoding this transporter, which predicts a protein of 573 amino acids with 8–9 putative transmembrane α-helices that seems to be a member of a new family of transport molecules.
...
1
2
3
...