Inhibition of the Renin-Angiotensinogen Reaction by Pepstatin

  title={Inhibition of the Renin-Angiotensinogen Reaction by Pepstatin},
  author={Franz Gross and J. Lazar and H. Orth},
  pages={656 - 656}
Pepstatin, an N-acylated pentapeptide obtained from culture filtrates of actinomycetes and first characterized as an inhibitor of pepsin, produces inhibition of the renin-substrate reaction both in vitro and in vivo. 
Inhibition of human plasma renin activity by pepstatin.
Pepstatin, a pentapeptide isolated from streptomyces, is a powerful inhibitor of several acid proteases. Its ability to inhibit the renin-angiotensinogen reaction was studied in vitro, in various
Effects of the renin inhibitor pepstatin on the blood pressure on intact and nephrectomized rats
It is suggested that pepstatin affects the reaction of both endogenous and exogenous heterologous renin with plasma substrate, and has a slight non-specific hypotensive action, as demonstrated in the nephrectomized rat.
Aldehydic peptides inhibiting renin
Aldehydic peptides in which the C terminal residue is leucinal or phenylalaninal were synthesized and appeared to be precursors of transition state analogues for renin‐catalysed amide bond hydrolysis.
Inhibition of pseudorenin by pepstatin.
Abstract The pentapeptide pepstatin was found to inhibit the ability of rat spleen pseudorenin to form angiotensin I from tetradecapeptide renin substrate. Dixon and Webb plots showed that this
Pentapeptide (Pepstatin) Inhibition of Brain Acid Proteinase
Pepstatin must be regarded as the most powerful agent yet described that inhibits intracellular acidic proteolytic enzyme in brain.
Inhibition of the local hemorrhagic Shwartzman reaction by an acid proteinase inhibitor, pepstatin
Nachweis, dass das lokaleShwartzman-Phänomen in Kaninchen durch intradermale oder i.v. Gabe von Pepstatin, einem sauren Proteinase-Hemmer, oder von Sojabohnen-Trypsin-Hemmer kurz vor der auslösenden
Pepstatin inhibits the digestion of hemoglobin and protein-polysaccharide complex by cathepsin D.
  • J. Woessner
  • Chemistry, Medicine
    Biochemical and biophysical research communications
  • 1972
Abstract Pepstatin, a peptide inhibitor of pepsin isolated from cultures of Actinomycetes, is shown to be a powerful inhibitor of cathepsin D preparations obtained from bovine uterus and rabbit ear
Effect of several proteinase inhibitors on renin reaction.
Inhibitory effect of the following nine proteinase inhibitors on the renin activity was studied: Trasylol, pepstatin A, chymostatin, antipain and leupeptin, which inhibited renin in vitro.
One-step separation of human plasma angiotensinogen from renin.
Human plasma angiotensinogen was totally separated from renin by chromatography on a Sepharose-hexamethylenediamine-Pepstatin column and 95% of the enzyme was recovered with a 25-fold purification.
Kinetics of the Inhibitory Effect of Pepstatin on the Reaction of Hog Renin with Rat Plasma Substrate
The reaction between hog renin and rat angiotensinogen followed Michaelis-Menten kinetics and pepstatin inhibited the reaction in a noncompetitive way, since renin activity could be restored by 24 hours of dialysis against distilled water.


A micromethod for measurement of renin in the plasma and kidney of rats.
A new micromethod for the measurement of renin activity and substrate in 0.1 ml of rat plasma and 1 mg of rat renal tissue is described, which permits repeated measurements of renIn activity in rats during acute experiments and in any physiological procedure.
0. Carretero and F. Gross, Naunyn-Schnledebergs Arch
  • Recent Progr. Hormone Res
  • 1965
We thank Professor H. Umezawa for the pepstatin and H. Malorny and U. Miksche for technical assistance