Inhibition of protein phosphorylation by opioid-inhibited adenylyl cyclase in rat brain membranes.

@article{Fleming1992InhibitionOP,
  title={Inhibition of protein phosphorylation by opioid-inhibited adenylyl cyclase in rat brain membranes.},
  author={L M Fleming and Gabri{\"e}lle A E Ponjee and Steven R. Childers},
  journal={The Journal of pharmacology and experimental therapeutics},
  year={1992},
  volume={260 3},
  pages={1416-24}
}
Opioid inhibition of adenylyl cyclase is a major second messenger system associated with opioid receptors in brain. To identify membrane phosphoproteins whose phosphorylation state is modulated by opioid inhibition of adenylyl cyclase, rat striatal membranes were preincubated with opioid agonists in the presence of 500 microM 5'-adenylyl-imidodiphosphate (which acted as a substrate for adenylyl cyclase, but not for protein kinase) before addition of [gamma-32P]ATP. Under these conditions… CONTINUE READING