Inhibition of protein, glycoprotein, ribonucleic acid and deoxyribonucleic acid synthesis by D-glucosamine and other sugars in mouse leukemic cells L5178Y and selective inhibition in SV-3T3 compared with 3T3 cells.

@article{Bosmann1971InhibitionOP,
  title={Inhibition of protein, glycoprotein, ribonucleic acid and deoxyribonucleic acid synthesis by D-glucosamine and other sugars in mouse leukemic cells L5178Y and selective inhibition in SV-3T3 compared with 3T3 cells.},
  author={H. Bruce Bosmann},
  journal={Biochimica et biophysica acta},
  year={1971},
  volume={240 1},
  pages={
          74-93
        }
}
  • H. Bosmann
  • Published 17 June 1971
  • Biology, Medicine
  • Biochimica et biophysica acta
Abstract d -Glucosamine inhibited DNA, RNA, protein and glycoprotein synthesis in L5178Y mouse leukemia cells at levels from 10–100 μmoles/ml. d -Glucosamine was the most inhibitory monosaccharide studied but other monosaccharides and derivatives at 100 μmoles/ml caused the following percent inhibition of protein synthesis: d -glucosamine, 95 %; UDP-glucose, 89 %; UDP- N -acetylglucosamine, 83 %; glucosamine 6-phosphate, 54 %; mannosamine 45 %; mannose, 45 %; galactose 24 %; galactosamine, 23… Expand
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SYNTHESIS OF GLYCOPROTEIN, GLYCOLIPID, PROTEIN, AND LIPID IN SYNCHRONIZED L5178Y CELLS
TLDR
Cell protein was found to be synthesized throughout the cell cycle, with the highest synthesis occurring in the S period; synthesis was depressed in the M period; and secreted glycoprotein was similar to secreted protein. Expand
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Inhibition of Glycoprotein Synthesis in L5178Y Mouse Leukaemic Cells by L-Asparaginase in vitro
TLDR
The preparation of L-asparaginase was free of contaminating enzymatic activity, although it was slightly active, and the synthesis of carbohydrate–amino-acid linkages in glycoproteins which involve the amide group of asparagine and N-acetyl-glucosamine residues was investigated. Expand
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