Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin

@article{Fenteany1995InhibitionOP,
  title={Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin},
  author={G. Fenteany and R. Standaert and William Arbuthnot Sir Lane and S. Choi and E. Corey and S. Schreiber},
  journal={Science},
  year={1995},
  volume={268},
  pages={726 - 731}
}
  • G. Fenteany, R. Standaert, +3 authors S. Schreiber
  • Published 1995
  • Biology, Medicine
  • Science
  • Lactacystin is a Streptomyces metabolite that inhibits cell cycle progression and induces neurite outgrowth in a murine neuroblastoma cell line. Tritium-labeled lactacystin was used to identify the 20S proteasome as its specific cellular target. Three distinct peptidase activities of this enzyme complex (trypsin-like, chymotrypsin-like, and peptidylglutamyl-peptide hydrolyzing activities) were inhibited by lactacystin, the first two irreversibly and all at different rates. None of five other… CONTINUE READING
    1,538 Citations

    Topics from this paper

    Lactacystin, Proteasome Function, and Cell Fate*
    • 365
    • PDF
    Inhibition of the 26S proteasome induces expression of GLCLC, the catalytic subunit for gamma-glutamylcysteine synthetase.
    • 51
    Towards subunit-specific proteasome inhibitors: synthesis and evaluation of peptide α', β'-epoxyketones
    • 131
    • Highly Influenced
    Substrate binding and sequence preference of the proteasome revealed by active-site-directed affinity probes.
    • 167
    • PDF
    Mechanistic Studies on the Inactivation of the Proteasome by Lactacystin in Cultured Cells*
    • 255
    • PDF
    Active Site-directed Inhibitors of Rhodococcus 20 S Proteasome
    • 53
    • PDF
    Catalytic activities of the 20 S proteasome, a multicatalytic proteinase complex.
    • 284

    References

    SHOWING 1-10 OF 35 REFERENCES
    The ubiquitin-proteasome proteolytic pathway
    • 1,664
    Proteasomes: protein and gene structures.
    • 94