Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin

  title={Inhibition of proteasome activities and subunit-specific amino-terminal threonine modification by lactacystin},
  author={G. Fenteany and R. Standaert and William Arbuthnot Sir Lane and S. Choi and E. Corey and S. Schreiber},
  pages={726 - 731}
  • G. Fenteany, R. Standaert, +3 authors S. Schreiber
  • Published 1995
  • Biology, Medicine
  • Science
  • Lactacystin is a Streptomyces metabolite that inhibits cell cycle progression and induces neurite outgrowth in a murine neuroblastoma cell line. Tritium-labeled lactacystin was used to identify the 20S proteasome as its specific cellular target. Three distinct peptidase activities of this enzyme complex (trypsin-like, chymotrypsin-like, and peptidylglutamyl-peptide hydrolyzing activities) were inhibited by lactacystin, the first two irreversibly and all at different rates. None of five other… CONTINUE READING
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