Inhibition of neutrophils' chemiluminescence by ethanol extract of propolis (EEP) and its phenolic components.


Ethanol extract of propolis (EEP), a natural beehive product, has been known for centuries for a variety of beneficial traditional medical properties, among which an anti-inflammatory effect is a major one. Now that most of its components have been isolated and recently identified, we tested 19 of them (all phenolic compounds) for their degree of anti-inflammatory activity. This was performed by evaluating the luminol-enhanced chemiluminescence, formed after their scavenging free radicals, generated by neutrophils that had been stimulated by phorbol myristate acetate. Caffeic-acid-phenylethyl-ester abolished the chemiluminescence completely at a concentration of 10 microM, while three flavone derivatives and three flavonols (galangin, kaempferol and kaempferid) diminished this chemiluminescence by 73-93% at the same concentration. These results indicate that some of the phenolic components of the ethanol extract of propolis are its active components in exerting its renowned anti-inflammatory activity.


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@article{Krol1996InhibitionON, title={Inhibition of neutrophils' chemiluminescence by ethanol extract of propolis (EEP) and its phenolic components.}, author={Wojciech L Krol and Scott Scheller and Zenon Pawel Czuba and Takao Matsuno and Grzegorz Zydowicz and Jashovam Shani and Magdalena Mos}, journal={Journal of ethnopharmacology}, year={1996}, volume={55 1}, pages={19-25} }