Inhibition of methionine uptake by methotrexate in mouse leukemia L1210 cells

  title={Inhibition of methionine uptake by methotrexate in mouse leukemia L1210 cells},
  author={K. Scanlon and A. Cashmore and M. Kashani-Sabet and M. Pallai and R. N. Dreyer and B. Moroson and M. Saketos},
  journal={Cancer Chemotherapy and Pharmacology},
  • K. Scanlon, A. Cashmore, +4 authors M. Saketos
  • Published 2004
  • Biology, Medicine
  • Cancer Chemotherapy and Pharmacology
  • SummaryMethionine-auxotrophic L1210 cells were used to study the effect of methotrexate (MTX) on methionine utake and metabolism. MTX was shown to inhibit amino acid transport systems and cause a decrease of methionine uptake into L1210 cells. Conversely, a nonmetabolizable amino acid analogue reduced MTX uptake into L1210 cells. MTX also blocked the transfer of the beta carbon from serine into methionine. Therefore, methionine deprivation may be an additional mechanism of action for MTX in… CONTINUE READING

    Figures and Topics from this paper.

    Molecular imaging of tumor metabolism and apoptosis
    • 18
    • Open Access


    Publications referenced by this paper.
    Carrier-mediated transport of the folic acid analogue, methotrexate, in the L1210 leukemia cell.
    • 349
    • Open Access
    The anticancer agent adriamycin can be actively cytotoxic without entering cells.
    • 557
    The regulation of folate and methionine metabolism.
    • 189
    • Open Access
    Neutral amino acid transport systems in Chinese hamster ovary cells.
    • 165
    • Open Access
    Intracellular concentration of sodium and other elements as related to mitogenesis and oncogenesis in vivo.
    • 216
    • Open Access