Inhibition of macrophage and endothelial cell nitric oxide synthase by diphenyleneiodonium and its analogs 1

@article{Stuehr1991InhibitionOM,
  title={Inhibition of macrophage and endothelial cell nitric oxide synthase by diphenyleneiodonium and its analogs 1},
  author={Dennis J. Stuehr and O A Fasehun and Nyoun Soo Kwon and Steven. S. Gross and Jos{\'e} Antonio Gonz{\'a}lez and Roberto Levi and Carl F. Nathan},
  journal={The FASEB Journal},
  year={1991},
  volume={5},
  pages={103 - 98}
}
The cofactor requirements of macrophage nitric oxide (NO·) synthase suggest involvement of an NADPH‐dependent flavoprotein. This prompted us to test the effect of the flavoprotein inhibitors diphenyleneiodonium (DPI), di‐2‐thienyliodonium (DTI), and iodoniumdiphenyl (ID) on the NO· synthases of macrophages and endothelium. DPI, DTI, and ID completely inhibited NO· synthesis by mouse macrophages, their lysates, and partially purified macrophage NO· synthase. Inhibition of NO· synthase by these… 

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