Inhibition of mRNA deadenylation and degradation by ultraviolet light.


Post-transcriptional mechanisms contribute to the changes in gene expression induced by cell stress. The effect of UV-B light on mRNA degradation in HeLa cells was investigated using a transcriptional chase system to determine the decay kinetics of tet-off vector-derived mRNAs containing or lacking a destabilizing AU-rich element. Degradation of both mRNAs was strongly inhibited in cells exposed to UV-B light. Removal of the poly(A)-tail, considered a crucial step in mRNA degradation, was strikingly impaired. UV light also inhibited deadenylation and degradation of endogenous mRNA of the chemoattractant cytokine interleukin (IL)-8. Both effects occurred rapidly and independently of newly induced genes. Importantly, stabilization of IL-8 mRNA was accompanied by a strong increase in the duration of IL-8 protein formation. Furthermore, general inhibition of protein synthesis, a hallmark of the response to cell stress, required far higher doses of UV-B than inhibition of mRNA deadenylation and degradation. The difference in sensitivity of cells to these effects of UV-B light establishes a dose range in which mRNA stabilization can lead to dramatically enhanced expression of proteins derived from normally unstable mRNAs, such as those of inflammatory cytokines, growth factors and proto-oncogenes, and thereby have a major impact on the response to UV light.


Citations per Year

201 Citations

Semantic Scholar estimates that this publication has 201 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Gowrishankar2005InhibitionOM, title={Inhibition of mRNA deadenylation and degradation by ultraviolet light.}, author={Gayatri Gowrishankar and Reinhard Winzen and Frank Bollig and Beniam Ghebremedhin and Natalie Redich and Birgit Ritter and Klaus Resch and Michael Kracht and Helmut Holtmann}, journal={Biological chemistry}, year={2005}, volume={386 12}, pages={1287-93} }