Inhibition of lipopolysaccharide‐inducible nitric oxide synthase, TNF‐α and COX‐2 expression by sauchinone effects on I‐κBα phosphorylation, C/EBP and AP‐1 activation

  title={Inhibition of lipopolysaccharide‐inducible nitric oxide synthase, TNF‐$\alpha$ and COX‐2 expression by sauchinone effects on I‐$\kappa$B$\alpha$ phosphorylation, C/EBP and AP‐1 activation},
  author={Ae-Kyung Lee and Sang Hyun Sung and Young Choong Kim and Sang -Geon Kim},
  journal={British Journal of Pharmacology},
Sauchinone, a lignan isolated from Saururus chinensis (Saururaceae), is a diastereomeric lignan with cytoprotective and antioxidant activities in cultured hepatocytes. The effects of sauchinone on the inducible nitric oxide synthase (iNOS), tumor necrosis factor‐α (TNF‐α) and cyclooxygenase 2 (COX‐2) gene expression and on the activation of transcription factors, nuclear factor‐κB (NF‐κB), CCAAT/enhancer‐binding protein (C/EBP), activator protein‐1 (AP‐1) and cAMP‐response element‐binding… 

Inhibition of double-stranded RNA-induced inducible nitric oxide synthase expression by fraxinellone and sauchinone in murine microglia.

The results suggest that an anti-inflammatory effect by fraxinellone and sauchinone is mediated via blockade of the JNK-STAT1/3-iNOS signaling pathway in viral-infected microglia.

Eugenolol and Glyceryl-Isoeugenol Suppress LPS-Induced INOS Expression by Down-Regulating NF-κB and AP-1 through Inhibition of Mapks and AKT/IκBα Signaling Pathways in Macrophages

  • J. YehJ. Hsu S. Liou
  • Biology, Chemistry
    International journal of immunopathology and pharmacology
  • 2011
It is suggested that eugenolol and glyceryl-isoeugenol may provide therapeutic benefits for inflammatory diseases by down-regulating NF-κB and AP-1 through inhibition of MAPKs and Akt/IκBα signaling pathways.

Suppressive effects of nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression by Citrus reticulata extract in RAW 264.7 macrophage cells.

  • K. JungE. Ha S. Yim
  • Biology
    Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
  • 2007

4-Hydroxykobusin inhibits the induction of nitric oxide synthase by inhibiting NF-kappaB and AP-1 activation.

The findings suggest that the inhibition of LPS-induced NO formation by 4-hydroxykobusin is due to its inhibition of NF-kappaB and AP-1 activation.



The Induction of Cyclooxygenase-2 mRNA in Macrophages Is Biphasic and Requires both CCAAT Enhancer-binding protein β (C/EBPβ) and C/EBPδ Transcription Factors*

CREB, NF-κB, and both C/EBPβ and -δ are identified as key factors in coordinately orchestrating transcription from the COX-2 promoter in activated macrophages.

Transcriptional Activation of the Cyclooxygenase-2 Gene in Endotoxin-treated RAW 264.7 Macrophages*

Cyclooxygenase-2 (COX-2), the enzyme primarily responsible for induced prostaglandin synthesis, is an immediate early gene induced by endotoxin in macrophages. We investigated the cis-acting elements

Antioxidants differentially regulate activation of nuclear factor-kappa B, activator protein-1, c-jun amino-terminal kinases, and apoptosis induced by tumor necrosis factor: evidence that JNK and NF-kappa B activation are not linked to apoptosis.

Results suggest that hydroxyl radicals mediate TNF-induced apoptosis but not activation of NF-kappa B, AP-1, and JNK, and lipid peroxides are required for all the signals induced by TNF.

Inhibition of lipopolysaccharide-induced I-kappaB degradation and tumor necrosis factor-alpha expression by dimethyl-4,4'-dimethoxy-5,6,5',6'-dimethylene dioxybiphenyl-2,2'-dicarboxylate (DDB): minor role in hepatic detoxifying enzyme expression.

It is demonstrated that DDB may inhibit inflammatory responses in association with reduction of NF-kappaB activation through prevention of I-kappBalpha degradation and subsequent TNF-alpha production, but not with modulation of the detoxifying enzyme expression.

Regulation of lipopolysaccharide-mediated interleukin-1beta release by N-acetylcysteine in THP-1 cells.

Data indicate that N-acetyl-L-cysteine modulates interleukin-1kappa release by increasing levels of the homo- and heterodimeric forms of nuclear factor-kappaB.

Osteopontin Is Induced by Nitric Oxide in RAW 264.7 Cells

It is suggested that NO directly up‐regulates the endogenous OPN in macrophages stimulated with LPS and IFN‐gamma, which may represent a negative feedback system acting to reduce iNOS expression.

S-adenosylmethionine attenuates the lipopolysaccharide-induced expression of the gene for tumour necrosis factor alpha.

AdoMet lowered the amount of TNF secreted by LPS-stimulated murine macrophage cells in a dose-dependent manner and inhibited TNF release in RAW cells, indicating that AdoMet administration might exert its hepatoprotective effects at least in part by its inhibitory effect on expression of the gene for TNF.