Inhibition of human muscle-specific enolase by methylglyoxal and irreversible formation of advanced glycation end products

@article{Pietkiewicz2009InhibitionOH,
  title={Inhibition of human muscle-specific enolase by methylglyoxal and irreversible formation of advanced glycation end products},
  author={Jadwiga Pietkiewicz and Andrzej Gamian and Magdalena Maria Staniszewska and Regina Danielewicz},
  journal={Journal of Enzyme Inhibition and Medicinal Chemistry},
  year={2009},
  volume={24},
  pages={356 - 364}
}
Methylglyoxal (MG) was studied as an inhibitor and effective glycating factor of human muscle-specific enolase. The inhibition was carried out by the use of a preincubation procedure in the absence of substrate. Experiments were performed in anionic and cationic buffers and showed that inhibition of enolase by methylglyoxal and formation of enolase-derived glycation products arose more effectively in slight alkaline conditions and in the presence of inorganic phosphate. Incubation of 15… Expand
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