Inhibition of herpes simplex thymidine kinase gene expression by DNA methylation is an indirect effect.

@article{Buschhausen1985InhibitionOH,
  title={Inhibition of herpes simplex thymidine kinase gene expression by DNA methylation is an indirect effect.},
  author={G Buschhausen and M. Graessmann and A. Graessmann},
  journal={Nucleic acids research},
  year={1985},
  volume={13 15},
  pages={5503-13}
}
The biological activity of in vitro methylated HSV-TK DNA was analysed after microinjection into thymidine kinase negative rat 2 cells. It was found that the fully methylated DNA (Hpa II methylase) was as active as the non methylated control DNA for about 48 hours after injection. DNA reextraction experiments and blot analysis showed that DNA demethylation… CONTINUE READING