Inhibition of glycosylphosphatidylinositol anchor formation by mannosamine.

  title={Inhibition of glycosylphosphatidylinositol anchor formation by mannosamine.},
  author={Y. T. Pan and Tetsu Kamitani and Chidambaram Bhuvaneswaran and Youssef Hallaq and Christopher D. Warren and Edward T H Yeh and Alan David Elbein},
  journal={The Journal of biological chemistry},
  volume={267 29},
Many eucaryotic cell surface proteins are anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI), of which the core region is highly conserved from protozoa to mammalian cells. Previous studies (Lisanti, M. P., Field, M. C., Caras, I. W., Menon, A. K., and Rodiguez-Boulan, E. (1991) EMBO J. 10, 1969-1977) showed that mannosamine blocked the expression of a recombinant GPI-anchored protein in Madin-Darby canine kidney cells and converted this protein to an unpolarized secretory… Expand
Identification of a species‐specific inhibitor of glycosylphosphatidylinositol synthesis
The results suggest that despite the conserved core structure, the GPI biosynthetic machinery may be different enough between mammalian and protozoa to represent a target for anti‐protozoan chemotherapy. Expand
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Glucosamine Inhibits Inositol Acylation of the Glycosylphosphatidylinositol Anchors in IntraerythrocyticPlasmodium falciparum *
This is the first study to report the specific inhibition of GPI inositol acylation by glucosamine in eukaryotes and offers a strategy for the development of novel anti-malarial drugs. Expand
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The characteristics of GPI biosynthesis are currently being explored for the development of parasite-specific inhibitors and the recent and promising progress is reviewed. Expand
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The results of these studies revealed that the inhibition of either N-linked glycosylation or oligosaccharide synthesis for GPI-anchor addition could affect the synthesis and the distribution of sALP, but not the kinetics of the phosphatase reaction. Expand
Synthesis of the GPI Anchor
Progress is being made in understanding the role of the GPI-anchor intermediates in the plasma membrane and in revealing the mechanisms by which these glycolipids travel from their site of synthesis to the cell surface. Expand
Mannosamine can replace glucosamine in glycosylphosphatidylinositols of Plasmodium falciparum in vitro.
It was observed that mannosamine is incorporated in vitro into P. falciparum glycolipids, which possess a phosphate group, which is novel and not been described for any other cellular or parasitic system. Expand
Detection of Glycophospholipid Anchors on Proteins
This unit provides a general approach for detecting GPI‐anchored proteins and a protocol for separation of lipid moieties released from proteins is provided and base hydrolysis of proteins is also presented. Expand
GPI-anchor synthesis.
The glycosyl phosphatidylinositol (GPI) anchor of membrane proteins is widely distributed in eukaryotes and parasitic protozoa and could be a target for chemotherapy. Expand
Chapter 12 Inhibitors of GPI Biosynthesis
The recent and promising progress is reviewed in the field of GPI inhibition, which is different enough between humans and a wide range of eukaryotic pathogens to represent a rich seam of potential therapeutic targets. Expand