Inhibition of gentamicin uptake into cultured mouse proximal tubule epithelial cells by L-lysine.

@article{Kaunitz1993InhibitionOG,
  title={Inhibition of gentamicin uptake into cultured mouse proximal tubule epithelial cells by L-lysine.},
  author={Jonathan D Kaunitz and Vierka Cummins and Dennis Mishler and Glenn T. Nagami},
  journal={Journal of clinical pharmacology},
  year={1993},
  volume={33 1},
  pages={63-9}
}
Gentamicin uptake and toxicity was studied in a nontransformed cell line obtained from the S1 segment of the proximal tubule epithelium of a transgenic mouse. Cytotoxicity was assayed using the dye 3-(4,-5dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). Gentamicin uptake was assayed by a fluorescence polarization assay. No differences in toxicity were found among cells incubated for 4 hours in complete culture medium, enriched Kreb's buffer alone, or enriched Krebs' buffer with… CONTINUE READING