Inhibition of degranulation of polymorphonuclear leukocytes by angiogenin and its tryptic fragment.

@article{Tschesche1994InhibitionOD,
  title={Inhibition of degranulation of polymorphonuclear leukocytes by angiogenin and its tryptic fragment.},
  author={Harald Tschesche and Christine Kopp and Walter Hermann H{\"o}rl and U Hempelmann},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 48},
  pages={30274-80}
}
A degranulation inhibiting protein was purified to apparent homogeneity from plasma ultrafiltrates of patients with uremia using gel-permeation chromatography, ion-exchange chromatography, affinity chromatography on blue Sepharose, and ion-exchange chromatography on a Mono S HR 5/5 fast protein liquid chromatography column. The identity of the isolated degranulation inhibiting protein with angiogenin was demonstrated by amino acid sequence determination, immunoblotting, and identical inhibitory… CONTINUE READING

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