Inhibition of chicken leukocyte migration in vitro: a direct agarose plate assay.


Leukocyte migration inhibition (LMI) is a widely used in vitro correlate of delayed type hypersensitivity (DTH) in mammals. This report describes the development of a direct agarose LMI assay for studying DTH in avian species. Optimum demonstration of LMI was found with leukocytes isolated on a Ficoll-diatrizoate gradient solution. The agarose culture… (More)


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