Inhibition of aquaporin-4 expression in astrocytes by RNAi determines alteration in cell morphology, growth, and water transport and induces changes in ischemia-related genes.

@article{Nicchia2003InhibitionOA,
  title={Inhibition of aquaporin-4 expression in astrocytes by RNAi determines alteration in cell morphology, growth, and water transport and induces changes in ischemia-related genes.},
  author={Grazia Paola Nicchia and Antonio Frigeri and Grazia Maria Liuzzi and Maria Svelto},
  journal={FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
  year={2003},
  volume={17 11},
  pages={1508-10}
}
Recent studies indicate a key role of aquaporin (AQP) 4 in astrocyte swelling and brain edema and suggest that AQP4 inhibition may be a new therapeutic way for reducing cerebral water accumulation. To understand the physiological role of AQP4-mediated astroglial swelling, we used 21-nucleotide small interfering RNA duplexes (siRNA) to specifically suppress AQP4 expression in astrocyte primary cultures. Semiquantitative RT-PCR experiments and Western blot analysis showed that AQP4 silencing… CONTINUE READING

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