Inhibition of apoptosis in serum starved porcine embryonic fibroblasts.

  title={Inhibition of apoptosis in serum starved porcine embryonic fibroblasts.},
  author={Chang-Kyu Lee and Jorge A Piedrahita},
  journal={Molecular reproduction and development},
  volume={62 1},
In nuclear transplantation, serum starvation is a general method to synchronize donor cells at the quiescent stage (G(0)) of the cell cycle. However, serum starvation during culture of mammalian cells may induce cell death, especially through apoptosis, thus contributing to the low efficiency of nuclear transplantation. This study was performed to characterize apoptosis during serum starvation and to determine the effects of apoptosis inhibitors such as a protease inhibitor [alpha(2… CONTINUE READING