Inhibition of Transfer Messenger RNA Aminoacylation and trans-Translation by Aminoglycoside Antibiotics* 210

@article{Corvaisier2003InhibitionOT,
  title={Inhibition of Transfer Messenger RNA Aminoacylation and trans-Translation by Aminoglycoside Antibiotics* 210},
  author={S. Corvaisier and V. Bordeau and B. Felden},
  journal={The Journal of Biological Chemistry},
  year={2003},
  volume={278},
  pages={14788 - 14797}
}
Transfer messenger RNA (tmRNA) directs the modification of proteins of which the biosynthesis has stalled or has been interrupted. Here, we report that aminoglycosides can interfere with this quality control system in bacteria, termedtrans-translation. Neomycin B is the strongest inhibitor of tmRNA aminoacylation with alanine (K i value of ∼35 μm), an essential step duringtrans-translation. The binding sites of neomycin B do not overlap with the identity determinants for alanylation, but the… Expand
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References

SHOWING 1-10 OF 35 REFERENCES
Amino acid acceptor identity switch of Escherichia coli tmRNA from alanine to histidine in vitro.
TLDR
Results indicate that the first alanine residue of the tag-peptide is actually derived from that aminoacylated to tmRNA and is substitutable by other amino acids during the trans -translation processes. Expand
Emerging views on tmRNA‐mediated protein tagging and ribosome rescue
TLDR
An updated model of tmRNA mediated protein tagging and ribosome rescue in bacteria is presented and the importance of bacterial tmRNAs for survival, growth under stress, and pathogenesis is discussed. Expand
Complex of Transfer-messenger RNA and Elongation Factor Tu
TLDR
It is demonstrated that the interaction of tmRNA with the elongation factor Tu shows unexpected features and can be efficiently cross-linked with EF-Tu·GDP using a zero-length cross-link. Expand
Binding of tobramycin leads to conformational changes in yeast tRNAAsp and inhibition of aminoacylation
TLDR
The results indicate that aminoglycosides are biologically effective when their binding induces a shift in a conformational equilibrium of the RNA. Expand
Simultaneous and functional binding of SmpB and EF-Tu-TP to the alanyl acceptor arm of tmRNA.
TLDR
Kinetic analysis of functioning combined with band-shift experiments and structural probing demonstrate, that tmRNA may indeed form a multimeric complex with SmpB, S1 and EF-Tu-GTP, which leads to a considerably enhanced efficiency of the initial steps of trans-translation. Expand
Modulation of RNA function by aminoglycoside antibiotics
TLDR
Improvements in RNA structure determination recently provided first insights into the decoding site of the ribosome at high resolution and how aminoglycosides might induce misreading of the genetic code, which could possibly be used in applied science. Expand
Aminoacylation of RNA minihelices with alanine
TLDR
It is shown here that a synthetic hairpin minihelix can be enzymatically aminoacylated with alanine, and the introduction of this base pair into tRNACys or tRNAPhe enables both to acceptAlanine. Expand
Antibiotic inhibition of RNA catalysis: neomycin B binds to the catalytic core of the td group I intron displacing essential metal ions.
TLDR
How neomycin B binds to the intron RNA inhibiting splicing in vitro is reported, suggesting electrostatic interactions and competition with divalent metal ions. Expand
The biological roles of trans-translation.
The unique transfer-messenger RNA (tmRNA) molecule has been identified in all bacterial species examined, suggesting that its action confers an important survival advantage to bacteria. Acting bothExpand
Inhibition of RNase P RNA cleavage by aminoglycosides.
TLDR
The findings suggest that RNase P, which is an essential enzyme, is indeed a potential drug target that can be used to develop new drugs by using various aminoglycosides as lead compounds. Expand
...
1
2
3
4
...