Inhibition of TPO-induced MEK or mTOR activity induces opposite effects on the ploidy of human differentiating megakaryocytes.

@article{Guerriero2006InhibitionOT,
  title={Inhibition of TPO-induced MEK or mTOR activity induces opposite effects on the ploidy of human differentiating megakaryocytes.},
  author={Raffaella Guerriero and Isabella Parolini and Elvira Pelosi and Paola Samoggia and Eleonora Petrucci and Massimo Sargiacomo and Cristiana Chelucci and Marco Gabbianelli and Cesare Peschle},
  journal={Journal of cell science},
  year={2006},
  volume={119 Pt 4},
  pages={
          744-52
        }
}
The megakaryocyte is a paradigm for mammalian polyploid cells. However, the mechanisms underlying megakaryocytic polyploidization have not been elucidated. In this study, we investigated the role of Shc-Ras-MAPK and PI3K-AKT-mTOR pathways in promoting megakaryocytic differentiation, maturation and polyploidization. CD34+ cells, purified from human peripheral blood, were induced in serum-free liquid suspension culture supplemented with thrombopoietin (TPO) to differentiate into a virtually pure… CONTINUE READING