Inhibition of Rho-Kinase Leads to Rapid Activation of Phosphatidylinositol 3-Kinase/Protein Kinase Akt and Cardiovascular Protection

@article{Wolfrum2004InhibitionOR,
  title={Inhibition of Rho-Kinase Leads to Rapid Activation of Phosphatidylinositol 3-Kinase/Protein Kinase Akt and Cardiovascular Protection},
  author={S. Wolfrum and A. Dendorfer and Y. Rikitake and T. Stalker and Yulan Gong and R. Scalia and P. Dominiak and J. Liao},
  journal={Arteriosclerosis, Thrombosis, and Vascular Biology},
  year={2004},
  volume={24},
  pages={1842-1847}
}
Objective—Rho-Kinase activity is increased in cardiovascular diseases and in patients with cardiovascular risk factors. However, it is not known whether inhibition of Rho-kinase could lead to cardiovascular protection and, if so, by what mechanism. Methods and Results—In human endothelial cells, the Rho-kinase inhibitor, hydroxyfasudil (HF) (1 to 100 &mgr;mol/L), increased Akt serine-473 phosphorylation within 15 minutes, leading to a 2.2-fold and 4.0-fold increase in Akt kinase activity and… Expand
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